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SCC100 MCF-7/S0.5 Human Breast Cancer Cell Line

MCF-7/S0.5 is a tamoxifen-sensitive cell line established from the MCF-7 human breast cancer cell line by adaption to growth in low serum conditions.

MCF-7/S0.5 Human Breast Cancer Cell Line MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture
SCC100
≥1X10⁶ cells/vial  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Key Applications
CULT
Description
Catalogue NumberSCC100
DescriptionMCF-7/S0.5 Human Breast Cancer Cell Line
Alternate Names
  • MCF7 S0.5
    Human breast cancer cell line
Background InformationEstrogen receptor-positive breast cancer is the most common type of breast cancer. About 80% of all breast cancers express the estrogen receptor (ER+) and depend on estrogen to support the growth and spread of the cancer cells. Hormonal therapy, such as tamoxifen, may help slow or stop the growth of ER+ breast cancers by blocking the actions of estrogen. Primary or acquired tamoxifen resistance poses a major clinical challenge in the treatment of ER+ breast cancer. The MCF-7/S0.5 is the parental line from which many tamoxifen-resistant cell lines have been derived and is utilized as a parental control to its derivative cell lines in parallel experiments.

The presence of hormones in the serum required in tissue culture media complicates the demonstration of specific hormone and antihormone effects. The MCF-7/S0.5 human breast cancer cells have been adapted to long-term growth in low serum concentration (0.5% fetal bovine serum) by a stepwise reduction of the medium serum content from 5% to 0.5% FBS. MCF-7/S0.5 cells express both estrogen and progesterone receptors and are sensitive to tamoxifen.

References:
1. Briand, P., and Lykkesfeldt, A.E. (1984) Effect of estrogen and antiestrogen on the human breast cancer cell line MCF-7 adapted to growth at low serum concentrations. Cancer Res. 44(3): 1114-1119.
2. Lykkesfeldt, A.E., and Briand, P. (1986) Indirect mechanism of oestradiol stimulation of cell proliferation of human breast cancer cell lines. Br. J. Cancer 53(1): 29-35.
3. Madsen, M.W., Reiter, B.E., Lykkesfeldt, A.E. (1995) Differential expression of estrogen receptor mRNA splice variants in the tamoxifen resistant human breast cancer cell line, MCF-7/TAMR-1 compared to the parental MCF-7 cell line. Mol. Cell Endocrinol. 109(2): 197-207.
References
Product Information
HS Code3002 90 99
Quality LevelMQ100
Applications
ApplicationMCF-7/S0.5 is a tamoxifen-sensitive cell line established from the MCF-7 human breast cancer cell line by adaption to growth in low serum conditions.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
ConcentrationPlease refer to lot specific datasheet.
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMCF-7/S0.5 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
SCC100 04054839075803