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CBA017 InnoCyte™ Cell Migration Assay, 24-well plate

CBA017
Purchase on Sigma-Aldrich

概述

重要规格表

Detection Methods
Fluorometric

Products

产品目录编号包装 数量 / 包装
CBA017-1KITCN 玻璃瓶 1 kit
Description
OverviewA convenient assay for chemotaxis cell migration in 24-well plates. Cells migrate through a 8 μm pore to a feeder layer containing serum or other attractant. Migrated cells are quantitated using a cell-permeable fluorescent dye.
Catalogue NumberCBA017
Brand Family Calbiochem®
Materials Required but Not Delivered 1X PBS (sterile)
Cell culture medium (serum-free)
Pipettors with disposable tips
Fluorescence reader capable of measuring fluorescence in 96-well plates with a filter set of ~485 nm (excitation) and 520 nm (emission).
Hemocytometer and microscope for cell counting
Tissue culture facility
Forceps
References
ReferencesWells, A., et al. 2002. Acta Oncol. 41, 124.
Staff, A. C. 2001. Scand. J. Clin. Lab. Invest. 61, 257.
Eatock, M.M., et al. 2000. Cancer Treat. Rev. 26, 19.
Klinghoffer, R.A., et al. 1999. EMBO J. 18, 2459.
Sieg, D.J., et al. 1999. J. Cell Sci. 112, 2677.
Smilenov, L. B., et al. 1999. Science 286, 1172.
Burridge, K. et al. 1997. Trends Cell Biol. 7, 342.
Huttenlocher, A., et al. 1997. J. Biol. Chem. 272, 32719.
Price, J.T., et al. 1997. Crit. Rev. Biochem. Mol. Biol. 32, 175.
Lauffenburger, D.A., and Horowitz, A.F. 1996. Cell 84, 359.
Product Information
Detection methodFluorometric
Form12 Tests
Format24-well plate
Kit contains12 Sterile Cell Culture Inserts, Sterile 24-well Plate, Black Plate Strips, Cell Detachment Buffer, Fluorescent Dye, Latrunculin A (inhibitor), and a user protocol.
Quality LevelMQ100
Biological Information
Assay time3-5 h
Sample TypeAdherent cells
Physicochemical Information
Emission max.
Excitation max.
Product Usage Statements
Intended useThe InnoCyte™ Cell Migration Assay, 24-Well is suited for studying the effects of various drugs or agents on cell motility and for identifying chemoattractant agents. The Cell Culture Inserts have an 8-µm pore size membrane that is suitable for migration of epithelial, mesenchymal, and endothelial cell types. The membrane is not coated with an extracellular matrix protein, so is not suitable for haptotactic migration studies. The assay is not intended for leukocyte migration experiments; leucocytes require membranes with a pore size that is smaller than 8 µm.
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage +2°C to +8°C
Storage ConditionsUpon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.
Protect from Light Protect from light
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Supplemental Information
Kit contains12 Sterile Cell Culture Inserts, Sterile 24-well Plate, Black Plate Strips, Cell Detachment Buffer, Fluorescent Dye, Latrunculin A (inhibitor), and a user protocol.
Global Trade Item Number
产品目录编号 GTIN
CBA017-1KITCN 07790788053628