CBA017 Sigma-AldrichInnoCyte™ Cell Migration Assay, 24-well plate

Figure 1: Assay Principle

Figure 2: HT-1080 cell Migration

HT-1080 cells were allowed incubated in the presence or absence of serum and Latrunculin A Solution for 3 h at 37°C in a 6% CO₂ tissue culture incubator (chemotactic migration) as described in the Detailed Protocol. Approximately 150,000 cells were loaded into triplicate Cell Culture Inserts. Migratory cells were subsequently dislodged and labeled as described in the Detailed Protocol.

Figure 3: HeLa Cell Migration

HeLa cells were incubated in the presence or absence of serum for 3 and 20 h at 37°C in a 6% CO₂ tissue culture incubator. Approximately 150,000 cells were loaded into triplicate Cell Culture Inserts. Migratory cells were subsequently dislodged and labeled as described in the Detailed Protocol. Migration overnight results in a two-fold increase in the number of migratory cells. Background migration (absence of serum) is low at both time points.