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IPFL07810 Immobilon®-FL PVDF Membrane

10 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Immobilon®-FL PVDF Membrane MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting, Dot Blot
IPFL07810
10  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Key Applications
WB, DB
Description
Catalogue NumberIPFL07810
Trade Name
  • Immobilon
DescriptionImmobilon®-FL PVDF Membrane
Background InformationOverview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
References
Product Information
Detection methodFluorescent Chemifluorescence Chromogenic Chemiluminescence
Compatible Stains
  • Coomassie brilliant blue
  • Amido black
  • Ponceau-S red
  • CPTS
Filter CodeIPFL
Filter ColorWhite
Quality LevelMQ400
Applications
Application10 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
Key Applications
  • Western Blotting
  • Dot Blot
Biological Information
BSA Adsorption205 µg/cm²
Goat IgG Adsorption300 µg/cm²
Insulin Adsorption155 µg/cm²
WettabilityHydrophobic
Physicochemical Information
Pore Size0.45 µm
Dimensions
Filter SurfacePlain
Filter Dimensions7 cm x 8.4 cm
Materials Information
Chemistry
  • Hydrophobic Polyvinylidene Fluoride (PVDF)
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Packaging Information
Material Size10
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
IPFL07810 04053252372605

Documentation

Immobilon®-FL PVDF Membrane MSDS

职位

物料安全数据表 (MSDS) 

参考

参考概述公共医疗ID
Chronic central administration of valproic acid: Increased pro-survival phospho-proteins and growth cone associated proteins with no behavioral pathology.
Bates, Ryan C, et al.
Pharmacol. Biochem. Behav., 103: 237-44 (2012)  2012

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小册子

标题
SNAP i.d. 2.0 System Brochure
Western Blotting Tools

技术信息

标题
Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system
Protein Blotting Handbook: 6th Edition

海报

标题
Low Background Membrane for Fluorescent Protein Detection in Western Blotting

用户指南

标题
Immobilon® Block Noise Cancelling Reagents
Immobilon®-FL Transfer Membrane User Guide
User Guide - Immobilon® FL Transfer Membrane