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EZHIL8-100K Human IL-8 Conferma™ ELISA

This Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.

Human IL-8 Conferma™ ELISA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Detection Methods: Colorimetric Research Category: Inflammation & Immunology, Immune Response, Metabolism Research Sub-Category: Cytokines & Cytokine Receptors, Immune Response, Inflammation, Metabolism
EZHIL8-100K
96-well strip plate  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
IL-8 HELISAColorimetric
Description
Catalogue NumberEZHIL8-100K
DescriptionHuman IL-8 Conferma™ ELISA
Background InformationIL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
References
Product Information
Detection methodColorimetric
Precision, %
  • Inter-assay: <4.7% CV
  • Intra-assay: <4.0% CV
Quality LevelMQ200
Applications
ApplicationThis Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.
Key Applications
  • ELISA
Application Notes3 hour 45 minute assay, room temperature, 50µl of neat serum or plasma is recommened.
Biological Information
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Analytes Available
  • IL-8
Entrez Gene Number
Entrez Gene SummaryIL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
Gene Symbol
  • IL-8
  • NAF
  • GCP1
  • LECT
  • LUCT
  • NAP1
  • GCP-1
  • LYNAP
  • MDNCF
  • MONAP
  • NAP-1
  • SCYB8
UniProt Number
UniProt SummaryIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
Accuracy
  • Serum 70%-105% Av 94%
  • Plasma 77%-102% Av 85%
Physicochemical Information
Linearity of DilutionSerum Linearity: 94-106% Serum Parallelism: 109% Plasma Linearity: 105%
Standard Curve Range
  • 0.82pg/ml-200pg/ml
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size96-well strip plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
EZHIL8-100K 04065265645669