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70796 His•Tag® Monoclonal Antibody

70796
Purchase on Sigma-Aldrich

概述

Replacement Information

Products

产品目录编号包装 数量 / 包装
70796-3CN 塑胶安瓿;塑胶针药瓶 100 μg
70796-4CN 塑胶安瓿;塑胶针药瓶 3 μg
Description
Overview

The His•Tag® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His•Tag sequence encoded by many of the Novagen® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10-8 to 1 × 10-9 M) enables sensitive, specific detection of His•Tag fusion proteins at antibody concentrations of 0.1-0.2 µg/ml. The His•Tag Monoclonal Antibody binds to N-terminal, C-terminal, and internal His•Tag sequences. This antibody also detects the recombinant marker bands in the Perfect Protein™ and Trail Mix™ Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-µg package size provides enough purified antibody for 1000 ml of working solution.

* Not recommended for HRP-based detection of Trail Mix Western Markers.



Catalogue Number70796
Brand Family Novagen®
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
70796-3CN 04055977272512
70796-4CN 04055977272529

Documentation

His•Tag® Monoclonal Antibody MSDS

职位

物料安全数据表 (MSDS) 

His•Tag® Monoclonal Antibody 分析证书

标题批号
70796

引用

标题
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    TB283 His•Tag® Monoclonal Antibody