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14-593M HePTP Protein, 250 µg

N-terminal His6-tagged, recombinant human HePTP, amino acid residues 22-end, for use in Phosphatase Assays.

14-593M
250 µg  Also available in 10 μg size (cat#14-593) and in bulk (cat# 14-593-K).
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-593M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHePTP Protein, 250 µg
OverviewN-terminal His6-tagged, recombinant human HePTP, amino acid residues 22- end
References
Product Information
Quality LevelMQ300
Applications
ApplicationN-terminal His6-tagged, recombinant human HePTP, amino acid residues 22-end, for use in Phosphatase Assays.
Key Applications
  • Phosphatase Assay
Biological Information
Sourceexpressed in E. coli
Specific ActivityFor Specific Activity Data, refer to the Certificate of Analysis for individual lots of this enzyme
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The noncatalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • PTPN7
  • BPTP-4
  • LC-PTP
  • LPTP
  • HEPTP
  • PTPNI
Protein TargetHePTP
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTyr phosphatase
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P35236 # Protein phosphatase that acts preferentially on tyrosine-phosphorylated MAPK1. Plays a role in the regulation of T and B-lymphocyte development and signal transduction.
SIZE: 360 amino acids; 40529 Da
SUBUNIT: Monomer. Interacts with MAPK1, MAPK3 and several other MAP kinases.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed exclusively in thymus and spleen.
PTM: Phosphorylated on serine residues in resting T-cells. Phosphorylation increases upon exposure to stimuli that increase intracellular cAMP levels. Phosphorylation leads to dissociation of bound MAP kinases.
SIMILARITY: SwissProt: P35236 ## Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. & Contains 1 tyrosine-protein phosphatase domain.
Molecular Weight40.7kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by dephosphorylation of 100μM DiFMUP
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C from date of shipment
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-593) and in bulk (cat# 14-593-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-593M 04053252513329

Documentation

相关产品和应用

Alternative Packsize

产品目录编号 描述
14-593 HePTP Protein, 10 µg

产品族

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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种类

Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Phosphatases
Life Science Research > Proteins and Enzymes > Purified Phosphatases