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14-744 Haspin Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human Haspin, amino acids 471-end, expressed by baculovirus in Sf21 insect cells. For use in Kinase Assays.

Haspin Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-744
10 µg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-744
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHaspin Protein, active, 10 µg
OverviewN-terminal 6His-tagged, recombinant, human Haspin, amino acids 471-end, expressed by baculovirus in Sf21 insect cells.
References
Product Information
Presentation10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, human Haspin, amino acids 471-end, expressed by baculovirus in Sf21 insect cells. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceRecombinant enzyme expressed in Sf21 cells
Specific Activity2237U/mg, where one unit of Haspin activity is defined as 1 nmol phosphate incorporated into 500 μM RARTLSFAEPG per minute at 30°C with a final ATP concentration of 100 μM.
Entrez Gene Number
Gene Symbol
  • GSG2
  • H-Haspin
  • haspin
Protein Targethaspin
Purification MethodPurified using Ni2+/NTA agarose. Purity 83% by SDS-PAGE and Coomassie blue staining.
Target Sub-FamilyMisc
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q8TF76 # Required for normal alignment of chromosomes at metaphase. Phosphorylates histone H3 'Thr-3' during mitosis.
COFACTOR: Magnesium.
SIZE: 798 amino acids; 88460 Da
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines.
PTM: Autophosphorylated on both serine and threonine residues (By similarity). Phosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: Q8TF76 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily. & Contains 1 protein kinase domain.
Molecular Weight41.1 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 500 μM RARTLSFAEPG.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of Haspin.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-744 04053252670992

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Alternative Packsize

产品目录编号 描述
14-744M Haspin Protein, active, 250 µg

产品族

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种类

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases