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368044 Granzyme A, Human, Recombinant, E. coli

Granzyme A, Human, Recombinant, E. coli, is a trypsin-like serine protease secreted by cytotoxic T cells and natural killer cells. Induces cell death through caspase-independent mechanism.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

同义词: GzmA

368044
Purchase on Sigma-Aldrich

概述

Replacement Information

Products

产品目录编号包装 数量 / 包装
368044-10UGCN 塑胶安瓿;塑胶针药瓶 10 μg
Description
OverviewRecombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. A proapoptotic enzyme that induces cell death through caspase-independent mechanism, causing single-stranded DNA nicking and other apoptotic features including nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin and activate the thrombin receptor on neuronal cells and astrocytes. It also cleaves and activates interleukin-1β. Note: The M.W. is that of a homodimer.
Catalogue Number368044
Brand Family Calbiochem®
SynonymsGzmA
References
ReferencesFan, Z., et al. 2002. Mol. Cell. Biol. 22, 2810.
Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol. 20, 323.
Beresford, P.J., et al. 2001. J. Biol. Chem. 276, 43285.
Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol. 244, 68.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.
EC number3.4.21.78
FormLiquid
FormulationIn 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.
Quality LevelMQ100
Applications
ApplicationGranzyme A, Human, Recombinant, E. coli, is a trypsin-like serine protease secreted by cytotoxic T cells and natural killer cells. Induces cell death through caspase-independent mechanism.
Biological Information
Specific Activity≥100 units/µg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
368044-10UGCN 04055977192117

Documentation

Granzyme A, Human, Recombinant, E. coli MSDS

职位

物料安全数据表 (MSDS) 

Granzyme A, Human, Recombinant, E. coli 分析证书

标题批号
368044

参考

参考信息概述
Fan, Z., et al. 2002. Mol. Cell. Biol. 22, 2810.
Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol. 20, 323.
Beresford, P.J., et al. 2001. J. Biol. Chem. 276, 43285.
Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol. 244, 68.

小册子

标题
Caspases and other Apoptosis Related Tools Brochure
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-July-2008 RFH
SynonymsGzmA
DescriptionRecombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease found in the cytotoxic granules and secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is a pro-apoptotic enzyme that induces cell death through a caspase-independent mechanism. Granzyme A-induced apoptosis results in single-stranded DNA nicking, nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin, activate the thrombin receptor on neuronal cells and astrocytes, cleave and activate interleukin-1β, and cleave putative HLA-associated protein II (PHAP II). Recombinant human Granzyme A expressed in E. coli.
FormLiquid
FormulationIn 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.
EC number3.4.21.78
Specific activity≥100 units/µg protein
Unit definitionOne unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesFan, Z., et al. 2002. Mol. Cell. Biol. 22, 2810.
Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol. 20, 323.
Beresford, P.J., et al. 2001. J. Biol. Chem. 276, 43285.
Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol. 244, 68.