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70967 GeneJuice® Transfection Reagent

70967
Purchase on Sigma-Aldrich

概述

Replacement Information

Products

产品目录编号包装 数量 / 包装
70967-3CN 玻璃瓶 1 ml
70967-4CN 玻璃瓶 10 ml
70967-5CN 塑胶安瓿;塑胶针药瓶 0.3 ml
70967-6CN 玻璃瓶 5 ml

Cell lines transfected with GeneJuice® Transfection Reagent
Description
Overview""I use GeneJuice because it's ease-of-use, low toxicity and wide range of target cells""
Stuart Rulten. Research Fellow. University of Sussex

""Since we have been working with GeneJuice, we have saved a lot of time and money!""
Dr. Andrea Kress, Institute of Clinical and Molecular Virology, Erlangen, Germany

""I find that Genejuice works for a multitude of cell types with minimal optimization""
Caitriona Marie Lyons. PhD Student. University College Cork, Ireland

Transfection Overview and Reagents

Transfection is the process by which nucleic acids are introduced into mammalian cells. GeneJuice Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity for mammalian cells. Whereas many available transfection reagents are based on cationic lipid formulation, GeneJuice Transfection Reagent is composed of a nontoxic cellular protein and a small amount of a novel polyamine. GeneJuice Transfection Reagent enables highly efficient DNA transfer in both stable and transient transfections of eukaryotic cells and is ideal for high-throughput transfections in a multi-well plate format. The unique composition is compatible with both serum-containing and serum-free media, making media changes unnecessary during transfection experiments. Genejuice is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, lipofection, and complex formation with DEAE-dextran. The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates.
Cell Lines:Primary Cells:
10T1/2
293T
3T3 NIH
3T3 Swiss
3T3-L1
A204
A431
A549
alpha TC1-6
AR 42J
As4.1
AtT-20
B50
BC-1
BC-2
BC3
BCBL
BHK-21
C3H/10T1/2
C6
C2C12
Caco-2
Caki-1
Calpan-1
Calu-1
Calu-6
CCL-131
CFPAC-1
Chang Liver
CHO
CHO-7
CHO-IR
CHO-K1
COS-1
COS-7
CS-1
CV-1
Daudi
DDTI MF-2
DT40
ECV304
EL4
ES-E14TG2a
EVSCC17M
H9c2
HCT-116
HEK293
HeLa
HeLa B
HeLa T4
Hep 3B2.1-7
HepG2
Hepa 1-6
Ht-29
HTB-37
HTB-45
Huh-7
HUVEC
IC21
IEC-6
JEG-3
Jurkat
KB
L57-3-11
L-6
L-929
MA-10
McA-RH7777
MCF-7
MCF-10-2A
MDCK
Melanocyte
MG-63
Neuro 2A
Neuroblastoma
NRK
NT2/D1
OV-1063
OVCAR3
P4
P19
PC12
PA317
PAM212
PS-1
R2C
RAW 264.7
RBL-2H3
RMP-41
SAOS-2
SC-1
Schneider line2
SK-N-MC
SK-N-SH
SKOV3
STO
SW-480
SW-837
T3M4
TM4
U937
UCD
Vero
Aortic smooth muscle cells
Astrocytes
Angioblasts
Chondrocytes
Chromaffin cells
Epithelial cells:
  mammary
  prostate
  tracheal
Fibroblasts
Keratinocytes
Catalogue Number70967 Brand Family Novagen®
Features and benefits
  • Highly efficient DNA transfer for both stable and transient transfections
  • Minimal cellular toxicity
  • Compatibility with both serum-containing and serum-free media
  • Simple protocol—no need for media changes
  • Ideal for high-throughput transfection in a multi-well plate format


References
References
  • Koleva, R.I., et al. 2006. J. Biol. Chem. 281, 25110.
  • Product Information
    DeclarationGeneJuice® is a registered trademark of EMD Chemicals Inc.
    Quality LevelMQ100
    Applications
    Biological Information
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    R PhraseR: 11

    Highly flammable.
    S PhraseS: 7-16

    Keep container tightly closed.
    Keep away from sources of ignition - No Smoking.
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Blue Ice Only
    Toxicity Flammable
    Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
    Storage +2°C to +8°C
    Do not freeze Ok to freeze
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications
    Global Trade Item Number
    产品目录编号 GTIN
    70967-3CN 07790788052928
    70967-4CN 04055977256215
    70967-5CN 04055977256222
    70967-6CN 04055977255577

    Documentation

    GeneJuice® Transfection Reagent MSDS

    职位

    物料安全数据表 (MSDS) 

    GeneJuice® Transfection Reagent 分析证书

    标题批号
    70967

    参考

    参考信息概述
  • Koleva, R.I., et al. 2006. J. Biol. Chem. 281, 25110.
  • 小册子

    标题
    Bulk Product Guide
    Nano Juice™ Transfection Kit
    The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression
    Transfection: A Science and an Art

    引用

    标题
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