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14-306 GSK3β Protein, active, 10 µg

Active, N-terminal His6-tagged recombinant human GSK3β with an H350L mutation, for use in Kinase Assays.

GSK3β Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-306
10 µg  Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-306M when ordering the 250μg size.
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-306
Brand Family Upstate
Trade Name
  • Upstate
DescriptionGSK3β Protein, active, 10 µg
OverviewN-terminal His6-tagged recombinant human GSK3β with an H350L mutation
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal His6-tagged recombinant human GSK3β with an H350L mutation, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryGlycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]
Gene Symbol
  • GSK3B
Protein TargetGSK3β
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P49841 # Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.
SIZE: 420 amino acids; 46744 Da
SUBUNIT: Monomer (By similarity). Interacts with NIN and CABYR.
TISSUE SPECIFICITY: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
PTM: Phosphorylated by AKT1 and ILK1.
SIMILARITY: SwissProt: P49841 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein kinase domain.
Molecular Weight51kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of Phospho-Glycogen Synthase Peptide-2
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-306M when ordering the 250μg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-306 04053252409486

Documentation

GSK3β Protein, active, 10 µg MSDS

职位

物料安全数据表 (MSDS) 

GSK3β Protein, active, 10 µg 分析证书

标题批号
GSK-3#946;, active - D8JN013U-T D8JN013U-T
GSK-3#946;, active - D8JN013U-V D8JN013U-V
GSK-3#946;, active - D8JN013U-W D8JN013U-W
GSK-3#946;, active - D8JN013U-X D8JN013U-X
GSK-3#946;, active - D8JN013U-Y D8JN013U-Y
GSK-3#946;, active - D8JN013U-Z D8JN013U-Z
GSK-3ß, active - D8JN013UAA D8JN013UAA
GSK-3β, active - D8JN013FU D8JN013FU
GSK-3β, active - WAA0024-I WAA0024-I
GSK‐3β ,active - D8JN013UA-A D8JN013UA-A

参考

参考概述应用公共医疗ID
Ethanol impairs insulin-stimulated neuronal survival in the developing brain: role of PTEN phosphatase
Xu, J., et al
J Biol Chem, 278:26929-37 (2003)  2003

Kinase Assay12700235 12700235
Protein kinase involved in lung injury susceptibility: Evidence from enzyme isoform genetic knockout and in vivo inhibitor treatment
Wainwright, M. S., et al
Proc Natl Acad Sci U S A, 100:6233-8 (2003)  2003

Activation Assay12730364 12730364
Regulation and functions of the glycogen synthase kinase-3 subfamily.
Woodgett, J R
Semin. Cancer Biol., 5: 269-75 (1994)  1994

显示摘要 		7803763 7803763
The alpha-isoform of glycogen synthase kinase-3 from rabbit skeletal muscle is inactivated by p70 S6 kinase or MAP kinase-activated protein kinase-1 in vitro.
Sutherland, C and Cohen, P
FEBS Lett., 338: 37-42 (1994)  1994

显示摘要 		8307153 8307153

问与答

问题回答
Why is it important to shake the reaction mixture during the incubations?It is important to shake the reaction mixture during incubations so that the substrate and the enzyme get good interaction. If you do not have access to a shaking incubator, use a water bath for the incubations, removing the tubes regularly to vortex.

相关产品和应用

Alternative Packsize

产品目录编号 描述
14-306M GSK3β Protein, active, 250 µg

产品族

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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GSK-3 Antibodies, Proteins, Inhibitors

Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways.
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种类

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases