Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-December-2010 RFH
Description
Native fibronectin purified from pooled bovine plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
Form
Liquid
Formulation
In 150 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3.
Concentration Label
Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Protocol for Coating of Tissue Culture Plates with Fibronectin
This protocol is provided only as a guideline; optimal conditions should be determined as needed. This procedure is based on the use of 21 cm2 dishes and 1 mg fibronectin, which is a sufficient quantity to coat 10 dishes at 5 µg/cm2.
1. Thaw the fibronectin by placing the vial in a 37°C water bath until it is completely thawed. Be VERY careful during the thawing process. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.
2. After thawing, bring the solution to a final volume of 20 ml with sterile, serum-free medium that has been pre-heated to 37°C. This yields a fibronectin solution of 50 µg/ml.
3. Add 2 ml to each of 10 tissue culture dishes and swirl gently to completely coat the entrie growth surface.
4. Incubate the dishes at room temperature for ~45 min to permit binding of the fibronectin to the growth surfaces.
5. Tilt each dish and remove the fibronecting using a sterile pipette. Do not permit the pipette tip to disturb the growth surface.
6. Add the cell suspension in medium directly to the fibronectin-coated dishes and incubate under conditions appropriate to the cells.
CAS number
86088-83-7
Purity
single band by SDS-PAGE
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
