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Native endothelial cell growth supplement from bovine neural tissue. A cell culture-medium supplement screened for contamination by bacteria, fungi, and mycoplasma. Mitogenic activity thought to be physically, chemically, and biologically distinct from that of FGF. Acts as a superior cloning factor for the isolation and growth of hybridoma cells and may eliminate the need for feeder cell layers.
Catalogue Number
324845
Brand Family
Calbiochem®
Synonyms
ECGS
References
References
Blann, A.D. 1991. Cell Biol. Int. Rep.15, 493. Jassal, D., et al. 1991. In Vitro Cell. Dev. Biol.27A, 625. Tanswell, A.K., et al. 1991. J. Dev. Physiol.15, 199. Olander, J., et al. 1980. In Vitro16, 209.
Product Information
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered NaCl containing streptomycin sulfate.
growth-promoting activity confirmed with fetal bovine endothelial cells
Concentration Label
Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R Phrase
R: 20/21/22-61
Harmful by inhalation, in contact with skin and if swallowed. May cause harm to the unborn child.
S Phrase
S: 22-36/37/39-45
Do not breathe dust. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Harmful & Carcinogenic / Teratogenic
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Canadian export regulations
Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Blann, A.D. 1991. Cell Biol. Int. Rep.15, 493. Jassal, D., et al. 1991. In Vitro Cell. Dev. Biol.27A, 625. Tanswell, A.K., et al. 1991. J. Dev. Physiol.15, 199. Olander, J., et al. 1980. In Vitro16, 209.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
20-June-2008 RFH
Synonyms
ECGS
Description
Native endothelial cell growth supplement from bovine neural tissue. A cell-culture-medium supplement for the growth and maintenance of many cell types. Growth promoting activities have been described for human endothelial cells, bovine endothelial cells, and mouse Balb/c fibroblasts. Can also substitute for feeder cells in support of the clonal growth of hybridomas. Mitogenic activity of the preparation is thought to be physically, chemically, and biologically distinct from that of FGF. Media containing 50-150 μg/ml is recommended for culturing vascular endothelial cells. Acts as a superior cloning factor for the isolation and growth of hybridoma cells and may eliminate the need for feeder cell layers. Screened for contamination by bacteria, fungi, and mycoplasma.
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered NaCl containing streptomycin sulfate.
Concentration Label
Please refer to vial label for lot-specific concentration
Biological activity
growth-promoting activity confirmed with fetal bovine endothelial cells
Solubility
Using aseptic technique, add 1-5 ml sterile serum-free medium and solubilize by gentle aspiration with a sterile pipette.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Toxicity
Harmful & Carcinogenic / Teratogenic
References
Blann, A.D. 1991. Cell Biol. Int. Rep.15, 493. Jassal, D., et al. 1991. In Vitro Cell. Dev. Biol.27A, 625. Tanswell, A.K., et al. 1991. J. Dev. Physiol.15, 199. Olander, J., et al. 1980. In Vitro16, 209.
