Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

PMEF-CFX EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Strain CF1, Irradiated, Passage 3

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
PMEF-CFX
5 vials  5-6x106 ea
Purchase on Sigma-Aldrich

特卖

概述

Replacement Information

特卖

Interested in purchasing an entire lot of MEF’s?
Bulk sized lots of MEFs are available with lot sampling.
Order Today

重要规格表

Key Applications
Cult
Description
Catalogue NumberPMEF-CFX
Trade Name
  • Embryomax
  • Specialty Media
DescriptionEmbryoMax® Primary Mouse Embryonic Fibroblasts
OverviewThese PMEF cells were isolated from CF-1 mouse embryos at day 13 and mitotically inactivated by treatment with irradiation. The cells can be directly plated onto gelatin coated (Cat. No. ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels:

• 6 x 60mm dishes
• 2 x T75 flasks
• 2 x 6 well plates

Refer to Murine Embryonic Stem Cell Culture Manual for complete culture protocols and media formulations for PMEF cells.
Alternate Names
  • MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells, Irradiated MEFS
Background InformationThe EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
References
Product Information
Quality LevelMQ100
Applications
ApplicationPMEF, Strain CF1, Irradiated, Passage 3
Key Applications
  • Stem Cell Culture
Biological Information
Stem Cell Type
  • Human Embryonic Stem Cells
  • Mouse Embryonic Stem Cells
  • PMEFs
  • Induced Pluripotent Stem Cells
Cell Line Type
  • Primary Mouse Embryo Fibroblasts
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsVials should be stored in the vapor phase of liquid nitrogen.
Packaging Information
Material Size5 vials
Material Package5-6x106 ea
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
PMEF-CFX 04053252923661

Documentation

EmbryoMax® Primary Mouse Embryonic Fibroblasts MSDS

职位

物料安全数据表 (MSDS) 

EmbryoMax® Primary Mouse Embryonic Fibroblasts 分析证书

标题批号
EmbryoMax#174; PMEF P3 Strain CF-1, Irradiated - RD1303001-1 RD1303001-1
EmbryoMax#174; PMEF P3 Strain CF-1, Irradiated - RD1304013-1 RD1304013-1
EmbryoMax® PMEF P3 Strain CF-1, Irradiated Primary Mouse Embryo Fibroblasts - 2440297-1 2440297-1
EmbryoMax® PMEF P3 -2495246-1 2495246-1
EmbryoMax® PMEF P3 -2513168-1 2513168-1
EmbryoMax® PMEF P3 -2513168-3 2513168-3
EmbryoMax® PMEF P3 -2706658-1 2706658-1
EmbryoMax® PMEF P3 -2735010-1 2735010-1
EmbryoMax® PMEF P3 Strain CF-1, Irradiated - 2463121-1 2463121-1
EmbryoMax® PMEF P3 Strain CF-1, Irradiated - 2990407-2 2990407-2
Show more

技术信息

标题
The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling