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324831 EGF, Human, Recombinant, E. coli

EGF, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

同义词: Epidermal Growth Factor, Human, Recombinant, E. coli

324831
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概述

Replacement Information

重要规格表

Purity
≥95% by SDS-PAGE

Products

产品目录编号包装 数量 / 包装
324831-200UGCN 塑胶安瓿;塑胶针药瓶 200 μg
Description
OverviewRecombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Acts as a survival factor in preventing apoptosis.
Catalogue Number324831
Brand Family Calbiochem®
SynonymsEpidermal Growth Factor, Human, Recombinant, E. coli
References
ReferencesMerlo, G.R., et al. 1995. J. Cell Biol. 128, 1185.
Yoshida, T., et al. 1993. Brain Res. Dev. 76, 147.
Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 415.
Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
Downward, J., et al. 1984. Nature 311, 483.
Gray, A., et al. 1983. Nature 303, 722.
Scott, J., et al. 1983. Science 221, 236.
Cohen, S. 1960. Proc. Natl. Acad. Sci. USA 46, 302.
Product Information
CAS number62229-50-9
FormSolid
FormulationLyophilized from sterile-filtered PBS.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 100-400 pg/ml as measured by its ability to stimulate the ³H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU/μg EGF
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSKB4518000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
324831-200UGCN 04055977216189

Documentation

EGF, Human, Recombinant, E. coli MSDS

职位

物料安全数据表 (MSDS) 

EGF, Human, Recombinant, E. coli 分析证书

标题批号
324831

参考

参考信息概述
Merlo, G.R., et al. 1995. J. Cell Biol. 128, 1185.
Yoshida, T., et al. 1993. Brain Res. Dev. 76, 147.
Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 415.
Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
Downward, J., et al. 1984. Nature 311, 483.
Gray, A., et al. 1983. Nature 303, 722.
Scott, J., et al. 1983. Science 221, 236.
Cohen, S. 1960. Proc. Natl. Acad. Sci. USA 46, 302.
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-May-2008 RFH
SynonymsEpidermal Growth Factor, Human, Recombinant, E. coli
DescriptionRecombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to native human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Binding of EGF to its 170 kDa transmembrane receptor results in dimerization of the receptor, activation of the intrinsic receptor tyrosine kinase, and initiation of the complex signal transduction cascade that leads to cell proliferation and/or differentiation. Acts as a survival factor in preventing apoptosis.
FormSolid
FormulationLyophilized from sterile-filtered PBS.
CAS number62229-50-9
RTECSKB4518000
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU/μg EGF
Biological activityED₅₀ = 100-400 pg/ml as measured by its ability to stimulate the ³H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3.
SolubilityReconstitute to a concentration of ≥10 µg/ml in 10 mM acetic acid containing ≥0.1% HSA or BSA.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C.
Toxicity Standard Handling
Merck USA index14, 3527
ReferencesMerlo, G.R., et al. 1995. J. Cell Biol. 128, 1185.
Yoshida, T., et al. 1993. Brain Res. Dev. 76, 147.
Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 415.
Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
Downward, J., et al. 1984. Nature 311, 483.
Gray, A., et al. 1983. Nature 303, 722.
Scott, J., et al. 1983. Science 221, 236.
Cohen, S. 1960. Proc. Natl. Acad. Sci. USA 46, 302.