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371206 Dkk-1, His•Tag®, Mouse, Recombinant

371206
Purchase on Sigma-Aldrich

概述

重要规格表

Purity
≥95% by SDS-PAGE

Products

产品目录编号包装 数量 / 包装
371206-10UGCN 玻璃瓶 10 μg
Description
OverviewRecombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.
Catalogue Number371206
Brand Family Calbiochem®
References
ReferencesPinzone, J.J. et al. 2009. Blood 113, 517.
Li, J. et al. 2006. Bone 36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
Niehrs, C. 2006. Oncogene 25, 7469.
Verani, R. et al. 2006. J. Neurochem. 101, 242.
Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
Adamska, M. et al. 2004. Dev. Biol. 272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B. et al. 2002. Nature 417, 664.
Mao, B. et al. 2001. Nature 411, 321.
Glinka, A. et al. 1998. Nature 391, 357.
Product Information
FormLyophilized
FormulationLyophilized from 0.2 µm filtered PBS, BSA.
Quality LevelMQ100
Biological Information
Biological activityND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.
Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1.0 EU/µg protein
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Regulatory Review
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Global Trade Item Number
产品目录编号 GTIN
371206-10UGCN 04055977191783