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2114 Detergent-OUT™ Micro Kit

The Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions.

Detergent-OUT™ Micro Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Protein Clean-up
2114
1 kit  10 columns
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Key Applications
PCU
Description
Catalogue Number2114
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionDetergent-OUT™ Micro Kit
OverviewThe Chemicon Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions. Removes detergents without significant dilution of protein solution. Simply load the protein solution on the Detergent-OUT Spin column and collect detergent free protein solution. Micro columns are suitable for removing detergent from a maximum of 0.2 mL sample volume.
Materials Required but Not DeliveredCentrifuge

Centrifuge tubes
References
Product Information
Components
  • Detergent-OUT Columns-DTG-100X 10 Micro
  • Collection Tubes (2 mL) 10
Applications
ApplicationThe Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions.
Key Applications
  • Protein Clean-up
Application NotesPROTOCOL:

1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.

2. Buffer Equilibration: SpinOUT columns are supplied in deionized water. Equilibrate the column with a buffer of your choice, as follows. Note: Do not equilibrate the columns with Tris buffer. Apply about 0.5 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.

3. Place the column in a 2.0 mL micro-centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.

4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 1-2 minutes. Note: If your protein solution is in Tris buffer, exchange the protein solution buffer to phosphate or other buffer, using either a SpinOUT column or by dialysis.

5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2-8°C for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
Packaging Information
Material Size1 kit
Material Package10 columns
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
2114 04053252278426

Documentation

Detergent-OUT™ Micro Kit MSDS

职位

物料安全数据表 (MSDS) 

Detergent-OUT™ Micro Kit 分析证书

标题批号
DETERGENT-OUT KIT (MICRO) - 1984691 1984691
DETERGENT-OUT KIT (MICRO) - 2058351 2058351
DETERGENT-OUT KIT (MICRO) - 3170442 3170442
DETERGENT-OUT KIT (MICRO) - 3194175 3194175
DETERGENT-OUT KIT (MICRO) - 3253471 3253471
DETERGENT-OUT KIT (MICRO) - 3279774 3279774
DETERGENT-OUT KIT (MICRO) - 3291975 3291975
DETERGENT-OUT KIT (MICRO) - 3305000 3305000
DETERGENT-OUT KIT (MICRO) - 3386072 3386072
DETERGENT-OUT KIT (MICRO) - 3446869 3446869
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数据表

标题
DETERGENT-OUT KIT (MICRO)