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268298 DAPI, Dihydrochloride - CAS 28718-90-3 - Calbiochem

Cell-permeable DNA-binding dye.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

同义词: 4ʹ,6-Diamidino-2-phenylindole, 2HCl

268298
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概述

Replacement Information

重要规格表

CAS #Empirical Formula
28718-90-3C₁₆H₁₅N₅ · 2HCl

Products

产品目录编号包装 数量 / 包装
268298-10MGCN 塑胶安瓿;塑胶针药瓶 10 mg
Description
OverviewCell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H2O (Cat. No. 508741).
Catalogue Number268298
Brand Family Calbiochem®
Synonyms4ʹ,6-Diamidino-2-phenylindole, 2HCl
References
ReferencesMathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
Hotz, M.A., et al. 1994. Cytometry 15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
Miller, T., et al. 1993. Biotechniques 15, 1042.
Gray, J.W., et al. 1982. Science 238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
Russels, W.C., et al. 1975. Nature 253, 461.
Product Information
CAS number28718-90-3
ATP CompetitiveN
FormYellow solid
Hill FormulaC₁₆H₁₅N₅ · 2HCl
Chemical formulaC₁₆H₁₅N₅ · 2HCl
Hygroscopic Hygroscopic
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetBinds preferentially to DNA rich in adenine and thymine
Purity≥90% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSNL5995050
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
268298-10MGCN 04055977197785

Documentation

DAPI, Dihydrochloride - CAS 28718-90-3 - Calbiochem MSDS

职位

物料安全数据表 (MSDS) 

DAPI, Dihydrochloride - CAS 28718-90-3 - Calbiochem 分析证书

标题批号
268298

参考

参考信息概述
Mathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
Hotz, M.A., et al. 1994. Cytometry 15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
Miller, T., et al. 1993. Biotechniques 15, 1042.
Gray, J.W., et al. 1982. Science 238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
Russels, W.C., et al. 1975. Nature 253, 461.

小册子

标题
Caspases and other Apoptosis Related Tools Brochure

技术信息

标题
LentiBrite™ Lentiviral Biosensors for Fluorescent Cellular Imaging: Analysis of Autophagosome Formation
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-June-2008 RFH
Synonyms4ʹ,6-Diamidino-2-phenylindole, 2HCl
DescriptionFluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
FormYellow solid
CAS number28718-90-3
RTECSNL5995050
Chemical formulaC₁₆H₁₅N₅ · 2HCl
Structure formulaStructure formula
Purity≥90% by HPLC
SolubilityEthanol, H₂O (5 mg/ml), or Methanol
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesMathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
Hotz, M.A., et al. 1994. Cytometry 15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
Miller, T., et al. 1993. Biotechniques 15, 1042.
Gray, J.W., et al. 1982. Science 238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
Russels, W.C., et al. 1975. Nature 253, 461.