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HTS155M ChemiSCREEN™ Membrane Preparation Recombinant Human MC5 Melanocortin Receptor

Human MC5 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Uni Prot Number: P33032 Entrez Gene Number: NM_005913.1
HTS155M
200 units  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

SpeciesHost CellsProtein Target
Human Chem-1MC5
Description
Catalogue NumberHTS155M
Trade Name
  • ChemiScreen
  • Chemicon
DescriptionChemiSCREEN™ Membrane Preparation Recombinant Human MC5 Melanocortin Receptor
OverviewFull-length human MC5R cDNA encoding MC5
Background InformationThe melanocortin system consists of five seven-transmembrane spanning G-protein coupled receptors (MC1-MC5). Among the five members of the melanocortin receptor family, MC2 and MC5 are expressed in peripheral tissues. The MC2 receptor (ACTH receptor) is almost exclusively expressed in the adrenal cortex whereas MC5 is expressed in several organs including the adrenal cortex. Studies have shown that targeted disruption of the MC5R gene produced mice with a severe defect in water repulsion and thermoregulation caused by decreased production of sebaceous lipids (Chen et al., 1997). Data show a requirement for the MC5 in multiple exocrine glands for the production of numerous products, indicative of a coordinated system for regulation of exocrine gland function by melanocortin peptides. (Entwistle et al.,1990). Millipore's MC5 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of MC5. The membrane preparations exhibit a Kd of 1.3nM for [125I]-[Nle4, D-Phe7]-α-MSH (NDP-αMSH). With 0.3 nM [125I]-NDP-αMSH, 10µg/well MC5 Membrane Prep typically yields greater than 50-fold signal-to-background ratio.
References
Product Information
FormatMembranes
PresentationLiquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, and dispensed at 1 mL/vial. Vials were rapidly frozen, and stored at -80°C
Quality LevelMQ100
Applications
ApplicationHuman MC5 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Key Applications
  • Radioligand binding assay
  • GTPγS binding
Application NotesRadioligand binding assay and GTPγS binding.
Biological Information
Entrez Gene Number
Gene Symbol
  • MC5R
  • MC5-R
  • MC-2
GPCR ClassA
Host CellsChem-1
Protein TargetMC5
Target Sub-FamilyMelanocortin
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P33032 # Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins.
SIZE: 325 amino acids; 36601 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in the brain but not in the melanoma cells.
SIMILARITY: SwissProt: P33032 ## Belongs to the G-protein coupled receptor 1 family.
Incubation ConditionsRECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min,, then washed with 50mM HEPES, pH 7.4, 500mM NaCl. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, filtered and stored at 4°C.
Radioligand: [125I]-NDP-αMSH (Perkin Elmer#: NEX-352)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl, filtered and stored at 4°C.

One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 50-fold signal:background with 125I labeled NDP-αMSH at 0.3 nM
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Table 1. Signal:background and specific binding values obtained in a competition binding assay with MC5 Receptor membrane prep.
Product Usage Statements
10 µg/well
Signal:Background 147.3
Specific Binding (cpm) 15208.7


SPECIFICATIONS: 1 unit = 10 µg
Bmax for [125I]-NDP-αMSH binding: 4.3 pmol/mg protein
Kd for [125I]-NDP-αMSH binding: ~1.3 nM Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Packaging Information
Material Size200 units
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
HTS155M 04053252368240

Documentation

ChemiSCREEN™ Membrane Preparation Recombinant Human MC5 Melanocortin Receptor 分析证书

标题批号
CHEMISCREEN™ MEMBRANE PREPARATION RECOMBINANT HUMAN MC5 MELANOCORTIN RECEPTOR - 2349732 2349732

参考

参考概述公共医疗ID
Exocrine gland dysfunction in MC5-R-deficient mice: evidence for coordinated regulation of exocrine gland function by melanocortin peptides
Chen, W, et al
Cell, 91:789-98 (1997)  1997

9413988 9413988
Characterization of functional melanotropin receptors in lacrimal glands of the rat
Entwistle, M L, et al
Peptides, 11:477-83 ()  1990

2166277 2166277

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