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218785 Caspase-10, Human, Recombinant, E. coli

Caspase-10, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag® sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

同义词: Mch4, FLICE-2

218785
Purchase on Sigma-Aldrich

概述

Replacement Information

Products

产品目录编号包装 数量 / 包装
218785-3000UCN 塑胶安瓿;塑胶针药瓶 3000 u
Description
OverviewRecombinant, human caspase-10 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
M.W. 11,000 and 18,000 (heterodimer).
Catalogue Number218785
Brand Family Calbiochem®
SynonymsMch4, FLICE-2
References
ReferencesKang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.
Product Information
Activity≥50,000 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=368057">Cat. No. 368057</a>) per min at 25°C, pH 7.4.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥150,000 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
218785-3000UCN 04055977218763

Documentation

Caspase-10, Human, Recombinant, E. coli MSDS

职位

物料安全数据表 (MSDS) 

Caspase-10, Human, Recombinant, E. coli 分析证书

标题批号
218785

参考

参考信息概述
Kang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.

小册子

标题
Caspases and other Apoptosis Related Tools Brochure
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-September-2007 RFH
SynonymsMch4, FLICE-2
DescriptionRecombinant, human caspase-10 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4.
Purity≥95% by SDS-PAGE
Specific activity≥150,000 units/mg protein
Activity≥50,000 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-pNA (Cat. No. 368057) per min at 25°C, pH 7.4.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesKang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.