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SCT120 BioTracker 488 Green Nuclear Dye

Live cell imaging green nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.

SCT120
1 Kit  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberSCT120
DescriptionBioTracker 488 Green Nuclear Dye
OverviewThe nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell's genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.

The BioTracker 488 Green Nuclear Dye is a cell-membrane permeable green fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step, and it has low toxicity for live cell imaging. The dye is supplied with a vial of verapamil, an efflux pump inhibitor that may improve probe retention and live cell staining in certain cell types.

Note: BioTracker™ Nuclear Dyes also shows blue fluorescence in the DAPI channel, and may not be suitable for multicolor imaging with blue probes.

Spectral Properties
Absorbance: 500nm
Emission: 515nm
Alternate Names
  • Live cell imaging probe
References
Product Information
Components
  • 1) 1 vial of 50µL BioTracker 488 Green Nuclear Dye (1000X in DMSO) (CS224594)
  • 2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)
Detection methodFluorescence
PresentationLiquid
Applications
ApplicationLive cell imaging green nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceSpectral Properties
Absorbance: 500nm
Emission: 515nm
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker 488 Green Nuclear Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size1 Kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
SCT120 04054839566943