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14-835M Aurora B Protein, active, 250 µg

Active, His-tagged, recombinant, human Aurora B, full length & N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end, for use in Kinase Assays.

Aurora B Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-835M
250 µg  Also available in 1 μg size (cat#14-835) and in bulk (cat# 14-835-K).
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-835M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAurora B Protein, active, 250 µg
OverviewHis-tagged, recombinant, human Aurora B, full length and N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, His-tagged, recombinant, human Aurora B, full length & N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourcehuman Aurora B, human INCENP co-expressed by baculovirus in Sf21 insect cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryChromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM]
Gene Symbol
  • AIM-1
  • STK12
  • Aik2
  • IPL1
  • Aurora-B
  • ARK2
  • aurora-B
  • AIK2
  • AIM1
  • AurB
  • STK5
  • aurora-1
  • STK-1
  • AURKB
Protein TargetAurora-B
Purification MethodGlutathione-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q96GD4 # May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis.
COFACTOR: Magnesium.
SIZE: 344 amino acids; 39280 Da
SUBUNIT: Interacts with TACC1. Associates with RACGAP1 during M phase. Component of the CPC at least composed of BIRC5/survivin CDCA8/borealin, INCENP and AURKB/Aurora-B. Interacts with CDCA1 and KNTC2. Interacts with EVI5.
SUBCELLULAR LOCATION: Nucleus. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid- body.TISSUE SPECIFICITY: High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.DISEASE:SwissProt: Q96GD4 # Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
SIMILARITY: SwissProt: Q96GD4 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. & Contains 1 protein kinase domain.
Molecular WeightAurora B = 40 kDa
INCENP = 38 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 1 μg size (cat#14-835) and in bulk (cat# 14-835-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-835M 04053252675126

相关产品和应用

Alternative Packsize

产品目录编号 描述
14-835 Aurora B Protein, active, 10 µg

产品族

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种类

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases