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MABT856-100UG Anti-von Willebrand factor (vWF) Antibody, clone IIIE2.34

Anti-von Willebrand factor, clone IIIE2.34, Cat. No. MABT856, is a mouse monoclonal antibody that detects human von Willebrand factor and has been tested for use in Immunocytochemistry and Western blotting.

MABT856-100UG
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABT856-100UG
DescriptionAnti-von Willebrand factor (vWF) Antibody, clone IIIE2.34
Alternate Names
  • vWF
Background Informationvon Willebrand factor (UniProt: P04275; also known as vWF) is encoded by the VWF (also known as F8VWF) gene (Gene ID:7450) in human. vWF is a multimeric plasma glycoprotein that is synthesized by endothelial cells and plays an important in the maintenance of hemostasis. It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form, which is then cleaved into von Willebrand antigen 2 (aa 23-763) and vWF (aa 764-2813). vWF is known to promote adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. vWF also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Mutations in VWF gene are linked to von Willebrand diseases 1-3 that are common hemorrhagic disorders caused by impaired platelet aggregation. Two isoforms of vWF have been described that are produced by alternative splicing.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Applications
ApplicationAnti-von Willebrand factor, clone IIIE2.34, Cat. No. MABT856, is a mouse monoclonal antibody that detects human von Willebrand factor and has been tested for use in Immunocytochemistry and Western blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected von Willebrand factor (vWF) in lysates from Human umbilical vein endothelial cells (HUVEC).

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected von Willebrand factor (vWF) in Human umbilical vein endothelial cells (HUVEC).
Biological Information
ImmunogenProtease fragment of VWF binding to heparin sepharose from human plasma.
CloneIIIE2.34
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone IIIE2.34 is a mouse monoclonal antibody that detects human von Willebrand factor. It targets an epitope with in the VWFD3 domain.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • VWF
  • F8VWF
Purification MethodProtein A purified
UniProt Number
Molecular Weight~270 kDa observed, 309.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human platelets cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected von Willebrand factor (vWF) in human platelets cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABT856-100UG 04054839755248

Documentation

Anti-von Willebrand factor (vWF) Antibody, clone IIIE2.34 MSDS

职位

物料安全数据表 (MSDS) 

Anti-von Willebrand factor (vWF) Antibody, clone IIIE2.34 分析证书

标题批号
Anti-von Willebrand factor (vWF), clone IIIE2.34 - 3457397 3457397
Anti-von Willebrand factor (vWF), clone IIIE2.34 - 3787652 3787652
Anti-von Willebrand factor (vWF), clone IIIE2.34 - 3899609 3899609
Anti-von Willebrand factor (vWF), clone IIIE2.34 - 3991446 3991446
Anti-von Willebrand factor (vWF), clone IIIE2.34 - 4193557 4193557
Anti-von Willebrand factor (vWF), clone IIIE2.34 - Q3208238 Q3208238
Anti-von Willebrand factor (vWF), clone IIIE2.34 - Q3208328 Q3208328