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FCMAB104A4 Sigma-AldrichAnti-phospho H3 (Ser10) Antibody, clone 3H10 Alexa Fluor® 488

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 488, is a Mouse Monoclonal Antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as H3S10p,
is labeled with Alexa Fluor® 488 and validated for use in FC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Epigenetics & Nuclear Function, Apoptosis & Cancer Research Sub-Category: Histones, Cell Cycle, DNA Replication & Repair Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, HIST3H3, H3FT, H3T, MGC126886 UniProt Number: Q16695

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	FC	M	AlexaFluor®488	Monoclonal Antibody

Description
Catalogue Number	FCMAB104A4
Description	Anti-phospho H3 (Ser10) Antibody, clone 3H10 Alexa Fluor® 488
Alternate Names	H3 histone, family 3A, H3S10P Histone H3 (phospho S10) H3 histone, family 3A H3 histone, family 3B H3 histone, family 3B (H3.3B)
Background Information	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

References

Product Information
Format	AlexaFluor®488
Control	Jurkat Cells
Presentation	Purified mouse IgG1k conjugated to Alexa Fluor® 488 in PBS with 15mg/mL BSA and less than 0.09% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 488, is a Mouse Monoclonal Antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as H3S10p, is labeled with Alexa Fluor® 488 and validated for use in FC.
Key Applications	Flow Cytometry

Biological Information
Immunogen	A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3.
Epitope	Ser10
Clone	3H10
Host	Mouse
Specificity	Histone H3 phosphorylated at Ser10
Isotype	IgG1κ
Species Reactivity	Human
Species Reactivity Note	Human. Broad species cross-reactivity is expected.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_003493
Entrez Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Gene Symbol	HIST1H3A H3FA HIST1H3B H3FL HIST1H3C H3FC HIST1H3D H3FB HIST1H3E H3FD HIST1H3F H3FI HIST1H3G H3FH HIST1H3H H3FK HIST1H3I H3FF HIST1H3J H3FJ HIST3H3 H3FT H3T MGC126886
Modifications	Phosphorylation
Purification Method	Protein G Purified
UniProt Number	Q16695
UniProt Summary	FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 136 amino acids; 15508 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in testicular cells. DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity). SIMILARITY: SwissProt: Q16695 Belongs to the histone H3 family. ... hide »
Molecular Weight	17 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Flow Cytometry with Jurkat cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.

Packaging Information
Material Size	100 tests

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
FCMAB104A4	04053252671241

Documentation

Anti-phospho H3 (Ser10) Antibody, clone 3H10 Alexa Fluor® 488 MSDS

职位
物料安全数据表 (MSDS)

Anti-phospho H3 (Ser10) Antibody, clone 3H10 Alexa Fluor® 488 分析证书

标题	批号
Anti-phospho H3 (Ser10), clone 3H10 Alexa Fluor#174; 488 conjugate - NRG1678394	NRG1678394
Milli-Mark Anti-phospho H3 (Ser10), clone 3H10 Alexa Fluor#174; 488 conjugate - 1990432	1990432
Milli-Mark Anti-phospho H3 (Ser10), clone 3H10 Alexa Fluor#174; 488 conjugate - 2002199	2002199
Milli-Mark Anti-phospho H3 (Ser10), clone 3H10 Alexa Fluor#174; 488 conjugate - 2018474	2018474
Milli-Mark Anti-phospho H3 (Ser10), clone 3H10 Alexa Fluor#174; 488 conjugate - 2051958	2051958
Milli-Mark Anti-phospho H3 (Ser10), clone 3H10 Alexa Fluor#174; 488 conjugate - 2201537	2201537
Milli-Mark Anti-phospho H3 (Ser10), clone 3H10 Alexa Fluor#174; 488 conjugate - FCMAB104A4	FCMAB104A4
Milli-Mark Anti-phospho H3 (Ser10), clone 3H10 Alexa Fluor#174; 488 conjugate - JH1741007	JH1741007
Milli-Mark Anti-phospho H3 (Ser10), clone 3H10 Alexa Fluor#174; 488 conjugate - NG1840147	NG1840147
Milli-Mark Anti-phospho H3 (Ser10), clone 3H10 Alexa Fluor#174; 488 conjugate - NG1882871	NG1882871

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