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04-282 Anti-phospho-EGFR (Thr654) Antibody, clone 3F2

04-282
100 µg  
Purchase on Sigma-Aldrich

特卖

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ca, H, M, RELISA, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number04-282
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-EGFR (Thr654) Antibody, clone 3F2
Alternate Names
  • ERBB
  • ERBB1
References
Product Information
FormatPurified
Control
  • Includes PMA and pervanadate treated Hep2G cell lysate as a positive control
Presentation100 µg (0.1 mg/ml) of mouse monoclonal IgG1 in PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol
Quality LevelMQ100
Applications
ApplicationDetect phospho-EGFR (Thr654) using this Anti-phospho-EGFR (Thr654) Antibody, clone 3F2 validated for use in ELISA, IP & WB.
Key Applications
  • ELISA
  • Immunoprecipitation
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 654 in human EGFR
Clone3F2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes phosphorylated EGFR on Threonine 654
IsotypeIgG1
Species Reactivity
  • Canine
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • EGFR
  • ERBB1
  • ERBB
  • mENA
Modifications
  • Phosphorylation
Purification MethodSubsequent thiophilic adsorption and size exclusion chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
SIZE: 1210 amino acids; 134277 Da
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
Molecular Weight180 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at -20°C from date of shipment
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
04-282 04053252590153

Documentation

Anti-phospho-EGFR (Thr654) Antibody, clone 3F2 分析证书

标题批号
Anti-phospho-EGFR (Thr654), clone 3F2 2913492
Anti-phospho-EGFR (Thr654), clone 3F2 (Mouse monoclonal IgG1) - 0138S0102 0138S0102
Anti-phospho-EGFR (Thr654), clone 3F2 - 2433299 2433299
Anti-phospho-EGFR (Thr654), clone 3F2 - 0138S0102 0138S0102
Anti-phospho-EGFR (Thr654), clone 3F2 - 2070809 2070809
Anti-phospho-EGFR (Thr654), clone 3F2 - 2191916 2191916
Anti-phospho-EGFR (Thr654), clone 3F2 - 2949486 2949486
Anti-phospho-EGFR (Thr654), clone 3F2 - 3189217 3189217
Anti-phospho-EGFR (Thr654), clone 3F2 - 4079361 4079361
Anti-phospho-EGFR (Thr654), clone 3F2 - 4187084 4187084

参考

参考概述公共医疗ID
Phosphorylation of Thr654 but not Thr669 within the juxtamembrane domain of the EGF receptor inhibits calmodulin binding
Aifa, Sami, et al
Biochem Biophys Res Commun, 347:381-7 (2006)  2006

16793002 16793002
Platelet-derived growth factor mimics phorbol diester action on epidermal growth factor receptor phosphorylation at threonine-654.
Davis, R J and Czech, M P
Proc. Natl. Acad. Sci. U.S.A., 82: 4080-4 (1985)  1985

显示摘要
2987962 2987962
Protein kinase C phosphorylation of the EGF receptor at a threonine residue close to the cytoplasmic face of the plasma membrane
Hunter, T, et al
Nature, 311:480-3 ()  1984

6090944 6090944

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Life Science Research > Antibodies and Assays > Primary Antibodies