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ABE101-S Sigma-AldrichAnti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size

Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated on lysine 9. Also known as H3K9me1, this antibody has dot blot proven specificity, and has been validated in WB, ICC, PIA. Small pack size is ideal for screening.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, HIST3H3, H3FT, H3T, MGC126886 UniProt Number: Q16695

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC, PIA, DB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABE101-S
Replaces	07-395
Description	Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size
Alternate Names	H3K9me1 Histone H3 (monomethyl K9) H3 histone family, member T Histone 3, H3 Histone cluster 3, H3
Background Information	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

References

Product Information
Format	Affinity Purified
Control	HeLa acid extract lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated on lysine 9. Also known as H3K9me1, this antibody has dot blot proven specificity, and has been validated in WB, ICC, PIA. Small pack size is ideal for screening.
Key Applications	Western Blotting Immunocytochemistry Peptide Inhibition Assay Dot Blot
Application Notes	Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in HeLa and A431 cells. Peptide Inhibition Analysis: 1:10,000 dilution from a representative lot was used with HeLa acid extract for peptide block analysis.

Biological Information
Immunogen	KLH-conjugated branched peptide corresponding to human Histone H3 methylated at Lys9.
Epitope	Lys9
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes Histone H3 methylated at Lys9.
Species Reactivity	Human
Species Reactivity Note	Demonstrated to react with human. Broad species cross-reactivity is expected based on sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_003484.1
Entrez Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq].
Gene Symbol	HIST1H3A H3FA HIST1H3B H3FL HIST1H3C H3FC HIST1H3D H3FB HIST1H3E H3FD HIST1H3F H3FI HIST1H3G H3FH HIST1H3H H3FK HIST1H3I H3FF HIST1H3J H3FJ HIST3H3 H3FT H3T MGC126886
Modifications	Methylation
Purification Method	Affinity Purfied
UniProt Number	Q16695
UniProt Summary	FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 136 amino acids; 15508 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in testicular cells. DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity). SIMILARITY: Belongs to the histone H3 family.
Molecular Weight	~ 17 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HeLa acid extract lysate. Western Blot Analysis: 0.1 µg/ml of this antibody detected Histone H3 on 10 µg of HeLa acid extract lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	10 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ABE101-S	04053252919169

Documentation

Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size MSDS

职位
物料安全数据表 (MSDS)

Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size 分析证书

标题	批号
Anti-monomethyl-Histone H3 (Lys9), Trial Size - 3407275	3407275
Anti-monomethyl-Histone H3 (Lys9), Trial Size - Q2227364	Q2227364
Anti-monomethyl-Histone H3 (Lys9), Trial Size -2420553	2420553
Anti-monomethyl-Histone H3 (Lys9), Trial Size Polyclonal Antibody	2908578
Anti-monomethyl-Histone H3 (Lys9), Trial Size, Polyclonal Antibody	2839545

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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