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07-369-I Sigma-AldrichAnti-dimethyl Histone H3 Antibody (Lys36)

Anti-dimethyl Histone H3 Antibody (Lys36) is a highly specific rabbit polyclonal antibody, that targets Histone H3 & has been tested in western blotting, Dot Blot & Chromatin IP (ChIP).

Anti-dimethyl Histone H3 Antibody (Lys36) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695

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概述

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, DB, ChIP	Rb	Serum	Polyclonal Antibody

Description
Catalogue Number	07-369-I
Description	Anti-dimethyl Histone H3 Antibody (Lys36)
Alternate Names	Histone H3.1t H3/t/ H3t/ H3/g
Background Information	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

References

Product Information
Format	Serum
Presentation	Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-dimethyl Histone H3 Antibody (Lys36) is a highly specific rabbit polyclonal antibody, that targets Histone H3 & has been tested in western blotting, Dot Blot & Chromatin IP (ChIP).
Key Applications	Western Blotting Dot Blot Chromatin Immunoprecipitation (ChIP)
Application Notes	Dot Blot Analysis: A 1:10,000 dilution from a representative lot detected dimethyl Histone H3 (Lys36), but not unmethylated Histone H3 or other peptides corresponding to modified histones. Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot imunoprecipitated dimethyl Histone H3 (Lys36) in HeLa cell lysate.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to a region near the N-terminus of human dimethyl Histone H3 methylated at Lys36.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Species Reactivity	Human
Species Reactivity Note	Predicted to react with Vertebrates based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_003484
Gene Symbol	HIST3H3 H3FT
Purification Method	Unpurified
UniProt Number	Q16695
Molecular Weight	~17 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HeLa acid extract. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected dimethyl Histone H3 (Lys36) in 10 µg HeLa acid extract and demonstrated a loss of signal in 0.5 µg of unmethylated recombinant Histone H3.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
07-369-I	04053252918643

Documentation

Anti-dimethyl Histone H3 Antibody (Lys36) MSDS

职位
物料安全数据表 (MSDS)

Anti-dimethyl Histone H3 Antibody (Lys36) 分析证书

标题	批号
Anti-dimethyl Histone H3 (Lys36)	2475921
Anti-dimethyl Histone H3 (Lys36)	3072328
Anti-dimethyl Histone H3 (Lys36) - 3193855	3193855
Anti-dimethyl Histone H3 (Lys36) - 3425572	3425572
Anti-dimethyl Histone H3 (Lys36) - 3856698	3856698
Anti-dimethyl Histone H3 (Lys36) - 4131166	4131166
Anti-dimethyl Histone H3 (Lys36) - Q2226882	Q2226882
Anti-dimethyl Histone H3 (Lys36) -2522242	2522242
Anti-dimethyl Histone H3 (Lys36) -2550070	2550070
Anti-dimethyl Histone H3 (Lys36) -2581791	2581791

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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