Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AB5421-I Anti-Vesicular Glutamate Transporter 3 (VGLUT3) Antibody

Anti-Vesicular Glutamate Transporter 3 (VGLUT3) is an antibody against Vesicular Glutamate Transporter 3 (VGLUT3) for use in Immunocytochemistry and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: Slc17a8, Vglut3 UniProt Number: Q7TSF2
AB5421-I
100 µL  
Purchase on Sigma-Aldrich

特卖

概述

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICCGpSerumPolyclonal Antibody
Description
Catalogue NumberAB5421-I
ReplacesAB5421
DescriptionAnti-Vesicular Glutamate Transporter 3 (VGLUT3) Antibody
Alternate Names
  • Vesicular glutamate transporter 3
  • Solute carrier family 17 member 8
  • VGluT3
Background InformationVesicular glutamate transporter 3 (UniProt Q7TSF2; also known as Solute carrier family 17 member 8, VGluT3) is encoded by the Slc17a8 (also known as Vglut3) gene (Gene ID 266767) in rat species. Vesicular glutamate transporters (VGluTs) are proton-dependent carriers responsible for the upload of the major excitatory neurotransmitter glutamate into synaptic vesicles within presynaptic terminals to allow later regulated release at the synaptic cleft. Studies conducted in mice show that VGluT1 and VGluT2 are essential for vital functions, while Vglut3-knockout mice have a normal life expectancy despite being deaf and developing anxiety and discrete basal locomotor phenotypes. VGluT1- and VGluT2-expressing neurons represent the bulk of canonical glutamatergic neurons in the brain, with VgluT1 representing the major subtype and accounting for approximately 80% of total vesicular transport of glutamate in the brain. In contrast, VgluT3 is found in discrete population of non-glutamatergic neurons using other transmitters than glutamate, such as cholinergic interneurons in the dorsal and ventral striatum, subpopulations of GABAergic basket cells in the cortex and hippocampus, and serotoninergic neurons, where it is involved in more subtle modulation of local transmission. Rat VGluT3 is a 12-transmembrane (a.a. 77-97, 131-151, 154-174, 183-203, 222-242, 250-270, 315-335, 354-374, 391-411, 414-434, 448-468, 482-502) protein with 7 cytoplasmic and 6 vacuolar domains, having both its N- and C-terminal tails (a.a. 1-76 and 503-585) exposed at the cytoplasmic side.
References
Product Information
FormatSerum
HS Code3002 15 90
PresentationGuinea pig polyclonal antibody serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Vesicular Glutamate Transporter 3 (VGLUT3) is an antibody against Vesicular Glutamate Transporter 3 (VGLUT3) for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:250 dilution from a representative lot detected vesicular glutamate transporter 3 (VGluT3) in PC12 rat pheochromocytoma cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a C-terminal cytoplasmic domain sequence of rat vesicular glutamate transporter 3 (VGluT3).
EpitopeC-terminal cytoplasmic domain.
ConcentrationPlease refer to lot specific datasheet.
HostGuinea Pig
SpecificityThis polyclonal guinea pig antiserum was raised against a VGluT3 C-terminal cytoplasmic domain sequence conserved between rat and murine species and shares 96% homology with the corresponding human sequence.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Slc17a8
  • Vglut3
Purification MethodUnpurified
UniProt Number
Molecular Weight~61 kDa observed. 64.76 kDa (rat), 66.15 kDa (mouse), and 64.99/59.08 kDa (human isoform 1/2) calculated. A ~130 kDa central nervous system VGluT3 isoform has also been described (Munguba, G.C., et al. (2011). Mol. Vis. 17:413-419). Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected vesicular glutamate transporter 3 (VGluT3) in 10 µg of PC12 rat pheochromocytoma cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
AB5421-I 04055977350166

Documentation

Anti-Vesicular Glutamate Transporter 3 (VGLUT3) Antibody MSDS

职位

物料安全数据表 (MSDS) 

Anti-Vesicular Glutamate Transporter 3 (VGLUT3) Antibody 分析证书

标题批号
Anti-Vesicular Glutamate Transporter -2803913 2803913
Anti-Vesicular Glutamate Transporter 3 (VGluT3) - 3212776 3212776
Anti-Vesicular Glutamate Transporter 3 (VGluT3) - 3821558 3821558
Anti-Vesicular Glutamate Transporter 3 (VGluT3) - 3921445 3921445
Anti-Vesicular Glutamate Transporter 3 (VGluT3) - 3956071 3956071
Anti-Vesicular Glutamate Transporter 3 (VGluT3) - 4092767 4092767
Anti-Vesicular Glutamate Transporter 3 (VGluT3) -2728274 2728274
Anti-Vesicular Glutamate Transporter 3 (VGluT3) -2768882 2768882
Anti-Vesicular Glutamate Transporter 3 (VGluT3) -2840400 2840400
Anti-Vesicular Glutamate Transporter 3 (VGluT3) -Q2679613 Q2679613
Show more