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			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

AB3042 Sigma-AldrichAnti-Uncoupling Protein 2 Antibody

This Anti-Uncoupling Protein 2 Antibody is validated for use in ELISA, WB for the detection of Uncoupling Protein 2.

Anti-Uncoupling Protein 2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
数据表

Research Category: Metabolism Research Sub-Category: Ion & Transport Channels Gene Symbol: UCP2, UCPH, SLC25A8 UniProt Number: P55851

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
M	ELISA, WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	AB3042
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Uncoupling Protein 2 Antibody
Alternate Names	UCP2

References

Product Information
Format	Affinity Purified
HS Code	3002 15 90
Presentation	Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA .
Quality Level	MQ100

Applications
Application	This Anti-Uncoupling Protein 2 Antibody is validated for use in ELISA, WB for the detection of Uncoupling Protein 2.
Key Applications	ELISA Western Blotting
Application Notes	Western Blotting: 1-10 μg/mL using Chemiluminescence technique. ELISA: 1:10,000-1:100,000; using 50-100 ng UCP2 control peptide/well. Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	14 aa peptide sequence at the C-terminus of the mouse UCP2 (Fleury et al. 1997). The peptide has no significant homology with UCP1.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Uncoupling Protein 2 (UCP2), mouse. Mouse UCP2 is a 309 amino acid mitochondrial uncoupling protein (Fleury et al. 1997). It is only 59% homologous with UCP1 found in brown adipose tissues. Mouse UCP2 (chromosome 7) has approximately 95% homology with human UCP2 located on chromosome 11 (Fleury et al. 1997). UCP2 has wide tissue distribution in mouse tissues (brain, kidney, liver, brown adipose tissue, heart, and muscle). UCP2 may play a critical role in energy balance, body weight, and thermoregulation (Fleury et al. 1997; Flier & Lowell 1997). SPECIES REACTIVITIES: The antibody has not yet been tested for cross-reactivity to other species. The sequence used for immunization shows 92% homology to rat and 86% homology to human UCP2.
Species Reactivity	Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_003355.2
Entrez Gene Summary	Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'.
Gene Symbol	UCP2 UCPH SLC25A8
UniProt Number	P55851
UniProt Summary	FUNCTION: SwissProt: P55851 # UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. SIZE: 309 amino acids; 33229 Da SUBUNIT: Acts as a dimer forming a proton channel (By similarity). SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle. SIMILARITY: SwissProt: P55851 ## Belongs to the mitochondrial carrier family. & Contains 3 Solcar repeats.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	50 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
AB3042	04053252274183

Documentation

Anti-Uncoupling Protein 2 Antibody MSDS

职位
物料安全数据表 (MSDS)

Anti-Uncoupling Protein 2 Antibody 分析证书

标题	批号
RABBIT ANTI-MOUSE UNCOUPLING PROTEIN 2 AFFINITY PURIFIED POLYCLONAL ANTIBODY	3045616
RABBIT ANTI-MOUSE UNCOUPLING PROTEIN 2 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2055978	2055978

参考

参考概述	公共医疗ID
Targeting deficiencies in mitochondrial respiratory complex I and functional uncoupling exerts anti-seizure effects in a genetic model of temporal lobe epilepsy and in a model of acute temporal lobe seizures. Simeone, KA; Matthews, SA; Samson, KK; Simeone, TA Experimental neurology 251 84-90 2014 显示摘要 Mitochondria actively participate in neurotransmission by providing energy (ATP) and maintaining normative concentrations of reactive oxygen species (ROS) in both presynaptic and postsynaptic elements. In human and animal epilepsies, ATP-producing respiratory rates driven by mitochondrial respiratory complex (MRC) I are reduced, antioxidant systems are attenuated and oxidative damage is increased. We report that MRCI-driven respiration and functional uncoupling (an inducible antioxidant mechanism) are reduced and levels of H2O2 are elevated in mitochondria isolated from KO mice. Experimental impairment of MRCI in WT hippocampal slices via rotenone reduces paired-pulse ratios (PPRs) at mossy fiber-CA3 synapses (resembling KO PPRs), and exacerbates seizure-like events in vitro. Daily treatment with AATP [a combination therapy composed of ascorbic acid (AA), alpha-tocopherol (T), sodium pyruvate (P) designed to synergistically target mitochondrial impairments] improved mitochondrial functions, mossy fiber PPRs, and reduced seizure burden index (SBI) scores and seizure incidence in KO mice. AATP pretreatment reduced severity of KA-induced seizures resulting in 100% protection from the severe tonic-clonic seizures in WT mice. These data suggest that restoration of bioenergetic homeostasis in the brain may represent a viable anti-seizure target for temporal lobe epilepsy.	24270080

参考概述

公共医疗ID

数据表

标题
RABBIT ANTI-MOUSE UNCOUPLING PROTEIN 2 AFFINITY PURIFIED POLYCLONAL ANTIBODY

产品目录编号	描述
AB3040	Anti-Uncoupling Protein 2 Antibody
AB3226	Anti-Uncoupling Protein 2 Antibody

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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