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662099 Anti-Ubiquitin Rabbit pAb

This Anti-Ubiquitin Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry, Immunoprecipitation for the detection of Ubiquitin.

Anti-Ubiquitin Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
662099
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityHostAntibody Type
B, Ca, Ch, Dr, E. coli, Fg, Gp, Ht, H, Mk, M, Rb, R, Sh, YeastRbPolyclonal Antibody

Products

产品目录编号包装 数量 / 包装
662099-50ULCN 塑胶安瓿;塑胶针药瓶 50 ul
Description
OverviewRecognizes the ~8.6 kDa unconjugated and various forms of conjugated ubiquitin.
Catalogue Number662099
Brand Family Calbiochem®
Application Data
Detection of ubiquitin protein by immunoblotting. Sample: Ubiquitin protein. Primary antibody: Anti-Ubiquitin Rabbit pAb (Cat. No. 662099) (1:5000). Detection: chemiluminescence.
References
ReferencesJungmann, J., et al. 1993. Nature 361, 369.
Hershko, A., and Ceichanover, A. 1992. Annu. Rev. Biochem. 61, 761.
Hochstrasser, M. 1992. Curr. Opin. Cell Biol. 4, 1024.
Jentsch, S. 1992. Annu. Rev. Genet. 26, 179.
Hershko, A., et al. 1982. J. Biol. Chem. 257, 13964.
Product Information
FormLiquid
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunohistochemistry
Immunoprecipitation
Application NotesImmunoblotting (1:1000, chemiluminescence)
Immunohistochemistry (1:200)
Immunoprecipitation (1:100)
Application CommentsVariables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogenbovine erytherocyte ubiquitin conjugated to KLH
ImmunogenBovine
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Canine
  • Chicken
  • Drosophila
  • E. coli
  • Frog
  • Guinea Pig
  • Hamster
  • Human
  • Monkey
  • Mouse
  • Rabbit
  • Rat
  • Sheep
  • Yeast
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C)
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
662099-50ULCN 04055977261189

Documentation

Anti-Ubiquitin Rabbit pAb MSDS

职位

物料安全数据表 (MSDS) 

Anti-Ubiquitin Rabbit pAb 分析证书

标题批号
662099

参考

参考信息概述
Jungmann, J., et al. 1993. Nature 361, 369.
Hershko, A., and Ceichanover, A. 1992. Annu. Rev. Biochem. 61, 761.
Hochstrasser, M. 1992. Curr. Opin. Cell Biol. 4, 1024.
Jentsch, S. 1992. Annu. Rev. Genet. 26, 179.
Hershko, A., et al. 1982. J. Biol. Chem. 257, 13964.

小册子

标题
Caspases and other Apoptosis Related Tools Brochure
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-August-2010 RFH
ApplicationImmunoblotting (1:1000, chemiluminescence)
Immunohistochemistry (1:200)
Immunoprecipitation (1:100)
Application Data
Detection of ubiquitin protein by immunoblotting. Sample: Ubiquitin protein. Primary antibody: Anti-Ubiquitin Rabbit pAb (Cat. No. 662099) (1:5000). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~8.6 kDa unconjugated and various forms of conjugated ubiquitin.
BackgroundUbiquitin is a highly conserved protein that functions in intracellular regulation. The ubiquitin system is the major pathway for selective protein degradation and is responsible for the degradation of abnormal and short-lived proteins. In the first step, ubiquitin is activated in an ATP-dependent reaction by ubiquitin-activating enzyme (E1). Activated ubiquitin is transferred to a ubiquitin conjugating enzyme (E2), then transferred to a protein substrate with the aid of ubiquitin-protein ligase (E3). An isopeptide linkage is formed between the C-terminus of the ubiquitin and an internal lysine residue of the substrate protein. Degradation requires the presence of a multi-ubiquitin chain on a substrate, which targets the protein for degradation by the 26S proteasome. Ubiquitin molecules are then released and recycled.
HostRabbit
Immunogen speciesBovine
Immunogenbovine erytherocyte ubiquitin conjugated to KLH
IsotypeIgG
SpeciesDrosophila, E. coli, bovine, canine, chicken, frog, guinea pig, hamster, human, monkey, mouse, rabbit, rat, sheep, yeast
FormLiquid
Preservative≤0.1% sodium azide
CommentsVariables associated with assay conditions will dictate the optimal working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C)
Toxicity Standard Handling
ReferencesJungmann, J., et al. 1993. Nature 361, 369.
Hershko, A., and Ceichanover, A. 1992. Annu. Rev. Biochem. 61, 761.
Hochstrasser, M. 1992. Curr. Opin. Cell Biol. 4, 1024.
Jentsch, S. 1992. Annu. Rev. Genet. 26, 179.
Hershko, A., et al. 1982. J. Biol. Chem. 257, 13964.