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05-829-AF555 Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 555 Conjugate

05-829-AF555
100 µL  
Purchase on Sigma-Aldrich

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概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, Gp, Av, B, Porcine, Gr, RICCMAlexaFluor®555Monoclonal Antibody
Description
Catalogue Number05-829-AF555
DescriptionAnti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 555 Conjugate
Alternate Names
  • Tubulin alpha-1 chain
  • Alpha-tubulin
  • Alpha-1 tubulin
Background InformationTubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tubes. Various post-translational modifications (PTMs), including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.
References
Product Information
FormatAlexaFluor®555
PresentationPurified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of α-Tubulin.
Key Applications
  • Immunocytochemistry
Application NotesThe unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.
Biological Information
ImmunogenPurified chicken brain α-tubulin.
EpitopeC-terminal 25 amino acids.
CloneDM1A
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).
IsotypeIgG1
Species Reactivity
  • Mouse
  • Human
  • Guinea Pig
  • Avian
  • Bovine
  • Porcine
  • Gerbil
  • Rat
Species Reactivity NoteMouse, Human, Guinea Pig, Avian, Bovine, Porcine, Gerbil, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TUBA1C
  • TUBA1
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~50 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-tubulin in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
05-829-AF555 04055977355550

Documentation

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 555 Conjugate MSDS

职位

物料安全数据表 (MSDS) 

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 555 Conjugate 分析证书

标题批号
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate - 3120123 3120123
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate - 3231926 3231926
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate - 3425404 3425404
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate - 3738006 3738006
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate - 4078570 4078570
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate - 4139150 4139150
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate -Q2696985 Q2696985