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ABE1003 Anti-Transportin-3 Antibody

This Anti-Transportin-3 Antibody is validated for use in Western Blotting for the detection of Transportin-3 .

Anti-Transportin-3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: TNPO3, IPO12 UniProt Number: Q9Y5L0
ABE1003
100 μg  
Purchase on Sigma-Aldrich

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概述

Replacement Information

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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1003
DescriptionAnti-Transportin-3 Antibody
Alternate Names
  • Transportin-3
  • Importin-12
  • Imp12
  • Transportin-SR
  • TRN-SR
  • Transportin-3
Background InformationTransportin 3 (UniProt Q9Y5L0; also known as Imp12, Importin 12, Limb girdle muscular dystrophy 1F, Transportin-SR, TRN-SR, TRN-SR2) is encoded by the TNPO3 gene (also known as IPO12, LGMD1F, MTR10A, TRNSR) (Gene ID 23534) in human. The transport of macromolecules between cytoplasm and nucleus is mediated by importins and exportins and is regulated by the small GTPase Ran. Importins bind their cargo in the cytoplasm, and the competitive binding of RanGTP induces cargoes release in the nucleus. Most nuclear importins and exportins, including transportin-3 (importin-12), belong to the beta-karyopherin family of proteins. Transportin-3 associates with and mediates the nuclear import of a wide range of serine/arginine-rich/RS domain-containing proteins, the majority of which are involved in mRNA processing, including the cleavage and polyadenylation specificity factor 6 (CPSF6, CF-Im-68) and the splicing factors alternative splicing factor/splicing factor 2 (ASF/SF2) & SC35. In addition, Transportin-3 also plays a cofactor role in facilitating HIV-1 replication. Phosphorylation of the ASF/SF2 RS domain is required for its interaction with Transprotin-3 HEAT repeat 15. Mutations in the HEAT repeat 15 region of Transprotin-3 likewise are reported to impair its interaction with CPSF6 and its ability to support HIV-1 replication.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Transportin-3 Antibody is validated for use in Western Blotting for the detection of Transportin-3 .
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Transportin-3.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityExpected to react with all four spliced isoforms.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Monkey, Mouse, Rat, Bovine, Equine, Ovine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TNPO3
  • IPO12
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~100 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Staurosporin treated Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Transportin-3 in 10 µg of Staurosporin treated Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABE1003 04055977173048