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ABE1440 Anti-Thymine-DNA Glycosylase Antibody

Anti-Thymine-DNA Glycosylase Antibody is an antibody against Thymine-DNA Glycosylase for use in Western Blotting, Immunocytochemistry.

Anti-Thymine-DNA Glycosylase Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: TDG UniProt Number: Q13569
ABE1440
100 µL  
Purchase on Sigma-Aldrich

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概述

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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1440
DescriptionAnti-Thymine-DNA Glycosylase Antibody
Alternate Names
  • G/T mismatch-specific thymine DNA glycosylase
  • hTDG
  • Thymine-DNA glycosylase
Background InformationG/T mismatch-specific thymine DNA glycosylase (EC 3.2.2.29; UniProt Q13569; also known as hTDG, Thymine-DNA glycosylase) is encoded by the TDG gene (Gene ID 6996) in human. Base excision repair (BER) is initiated by damage-specific DNA glycosylases that recognize and remove aberrant bases by hydrolyzing the N-glycosidic bond, creating an apurinic/apyrimidinic (AP) site. This is followed by an (AP)-endonuclease-catalyzed DNA strand incision 5′ to the AP site, trimming of the 5′ end by an (AP)-lyase or an endonuclease, DNA resynthesis by a DNA polymerase and strand sealing by a DNA ligase. Monofunctional glycosylases have only glycosylase activity, whereas bifunctional glycosylases possess additional AP lyase activity and can therefore convert a base lesion into a single-strand break without the need for a separate AP endonuclease. TDG is a monofunctional DNA glycosylase that excises thymine and uracil from G·T and G·U mismatched oligonucleotide substrates as well as the highly mutagenic adduct 3,N4-ethenocytosine from double-stranded DNA. TDG DNA-binding affinity is regulated by SUMOylation, which dramatically reduces its affinity toward DNA substrate and AP site. Sumoylation also potentiates the stimulatory effect of (AP)-endonuclease (APE1/HAP1) on TDG.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Thymine-DNA Glycosylase Antibody is an antibody against Thymine-DNA Glycosylase for use in Western Blotting, Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 2.5 µg/mL from a representative lot detected Thymine-DNA Glycosylase in A431, HeLa, HUVEC, and NIH/3T3 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a sequence from the N-terminal region of human Thymine-DNA Glycosylase.
EpitopeN-terminal
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TDG
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~55 kDa observed. 46.05 kDa calculated. 55–60 kDa unmodified and 81-86 kDa posttranslationally modified forms reported (Hardeland, U., et al. (2002). EMBO J. 21(6): 1456-1464). Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Thymine-DNA Glycosylase in 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABE1440 04055977329551