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654250 Anti-TNF-α Rabbit pAb

This Anti-TNF-α Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Neutralization Studies, Paraffin Sections, Radioimmunoassay for the detection of TNF-α.

Anti-TNF-α Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

同义词: Anti-Tumor Necrosis Factor-α

654250
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

Products

产品目录编号包装 数量 / 包装
654250-1MGCN 塑胶安瓿;塑胶针药瓶 1 mg
Description
OverviewRecognizes recombinant and monocyte-derived TNF-α. Exhibits some cross-reactivity with lymphotoxin (TNF-β) and mouse TNF-α in L929 cells.
Catalogue Number654250
Brand Family Calbiochem®
SynonymsAnti-Tumor Necrosis Factor-α
References
Product Information
Unit of Definition1 EU corresponds to 0.1 ng endotoxin (EC-6/USP Standard)
FormLiquid
FormulationIn PBS.
Positive controlL929 cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Neutralization Studies
Paraffin Sections
Radioimmunoassay
Application NotesELISA (see comments)
Frozen Sections (see comments)
Immunoblotting (see comments)
Neutralization Studies (see comments)
Paraffin Sections (see comments)
Radioimmunoassay (see comments)
Application CommentsReacts specifically with natural and recombinant human TNF-α. An approximately 20 µg/ml solution of this antibody has been shown to neutralize 100% of the activity of 0.5 ng/ml recombinant human TNF-α, as measured in the standard L929 cell bioassay. This antibody has also been reported to work for ELISA, frozen sections, immunoblotting, paraffin sections, radioimmunoassay. Endotoxin: ≤5 EU/mg protein as determined by the Biowhittaker Endotect LAL assay. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenrecombinant, human TNF-α
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsEndotoxins: ≤1 EU/mg
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
654250-1MGCN 04055977184617

Documentation

Anti-TNF-α Rabbit pAb MSDS

职位

物料安全数据表 (MSDS) 

Anti-TNF-α Rabbit pAb 分析证书

标题批号
654250
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2009 JSW
SynonymsAnti-Tumor Necrosis Factor-α
ApplicationELISA (see comments)
Frozen Sections (see comments)
Immunoblotting (see comments)
Neutralization Studies (see comments)
Paraffin Sections (see comments)
Radioimmunoassay (see comments)
DescriptionRabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes recombinant and monocyte-derived TNF-α.
BackgroundHuman Tumor Necrosis Factor α (TNF-α, cachectin) is a cytokine, produced by variety of cell types, that is involved in mediation of the inflammatory response, resistance to infection, and resistance to tumor growth. Human and mouse TNF-α are approximately 79% homologous at the amino acid level and exhibit cross-species reactivity. This antibody recognizes both recombinant and natural human TNF-α and exhibits some (~30%) cross-reactivity with mouse TNF-α. It exhibits only minor (<1%) cross-reactivity with human TNF-β (lymphotoxin).
HostRabbit
Immunogen speciesHuman
Immunogenrecombinant, human TNF-α
IsotypeIgG
Specieshuman, mouse (weakly)
Positive controlL929 cells
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
ContaminantsEndotoxins: ≤1 EU/mg
Unit definition1 EU corresponds to 0.1 ng endotoxin (EC-6/USP Standard)
PreservativeNone
CommentsReacts specifically with natural and recombinant human TNF-α. An approximately 20 µg/ml solution of this antibody has been shown to neutralize 100% of the activity of 0.5 ng/ml recombinant human TNF-α, as measured in the standard L929 cell bioassay. This antibody has also been reported to work for ELISA, frozen sections, immunoblotting, paraffin sections, radioimmunoassay. Endotoxin: ≤5 EU/mg protein as determined by the Biowhittaker Endotect LAL assay. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling