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AB770 Anti-TIMP-1 Antibody

AB770
100 µg  
Purchase on Sigma-Aldrich

特卖

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, Eq, Ft, R, Rb, ShELISA, WB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB770
Replaces04-1133
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-TIMP-1 Antibody
Alternate Names
  • Tissue Inhibitor of Metalloproteinase-1
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
PresentationPurified immunoglobulin. Liquid in PBS containing 0.02% azide.
Quality LevelMQ100
Applications
ApplicationAnti-TIMP-1 Antibody detects level of TIMP-1 & has been published & validated for use in ELISA, WB, IH.
Key Applications
  • ELISA
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting at 5-10 μg/mL: recognizes a band of approximately 28-30kDa in reduced samples.

Immunohistochemistry: frozen and 4%PFA fixed paraffin sections: 5-20μg/mL enzymatic detection.



Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenFull length purified human fibroblast tissue inhibitor of metalloproteinases {Clark, IM (1991) Matrix 11:76-85}.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityReacts with TIMP1. Does not react with any other human proteins tested so far. The antibody reacts with native and denatured TIMP1 and TIMP1 in and out of enzyme/inhibitor complex.
Species Reactivity
  • Bovine
  • Horse
  • Ferret
  • Rat
  • Rabbit
  • Sheep
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
Gene Symbol
  • TIMP1
  • TIMP
  • FLJ90373
  • CLGI
  • EPA
  • EPO
  • TIMP-1
  • HCI
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01033 # Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
SIZE: 207 amino acids; 23171 Da
SUBCELLULAR LOCATION: Secreted.
PTM: The activity of TIMP1 is dependent on the presence of disulfide bonds.
SIMILARITY: SwissProt: P01033 ## Belongs to the protease inhibitor I35 (TIMP) family. & Contains 1 NTR domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C for up to three months from date of receipt. For extended storage, freeze in convenient undiluted aliquots at -20°C for up to twelve months from date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
AB770 08436037124501

Documentation

Anti-TIMP-1 Antibody MSDS

职位

物料安全数据表 (MSDS) 

Anti-TIMP-1 Antibody 分析证书

标题批号
RABBIT ANTI-HUMAN TIMP-1 POLYCLONAL ANTIBODY - 2151835 2151835
RABBIT ANTI-HUMAN TIMP-1 POLYCLONAL ANTIBODY - 2436304 2436304
RABBIT ANTI-HUMAN TIMP-1 - 2488812 2488812
RABBIT ANTI-HUMAN TIMP-1 - 3245658 3245658
RABBIT ANTI-HUMAN TIMP-1 - 3455142 3455142
RABBIT ANTI-HUMAN TIMP-1 - 3488541 3488541
RABBIT ANTI-HUMAN TIMP-1 - 3598062 3598062
RABBIT ANTI-HUMAN TIMP-1 - 3770312 3770312
RABBIT ANTI-HUMAN TIMP-1 - 3942747 3942747
RABBIT ANTI-HUMAN TIMP-1 -2714558 2714558

参考

参考概述应用品种公共医疗ID
Remodeling of aorta extracellular matrix as a result of transient high oxygen exposure in newborn rats: implication for arterial rigidity and hypertension risk.
Huyard, F; Yzydorczyk, C; Castro, MM; Cloutier, A; Bertagnolli, M; Sartelet, H; Germain, N; Comte, B; Schulz, R; DeBlois, D; Nuyt, AM
PloS one  9  e92287  2014

显示摘要
ImmunohistochemistryRat24743169 24743169
Blockade of the MEK/ERK pathway with a raf inhibitor prevents activation of pro-inflammatory mediators in cerebral arteries and reduction in cerebral blood flow after subarachnoid hemorrhage in a rat model.
Maddahi, A; Ansar, S; Chen, Q; Edvinsson, L
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism  31  144-54  2011

显示摘要
20424636 20424636
Changes in the expression of MMP-3, MMP-9, TIMP-1 and aggrecan in the condylar cartilage of rats induced by experimentally created disordered occlusion.
Guo-Wei Wang,Mei-Qing Wang,Xiao-Jing Wang,Shi-Bin Yu,Xiao-Dong Liu,Kai Jiao
Archives of oral biology  55  2010

显示摘要
20728870 20728870
MMP-9 overexpression improves myogenic cell migration and engraftment.
Morgan J, Rouche A, Bausero P, Houssaïni A, Gross J, Fiszman MY, Alameddine HS
Muscle Nerve  42  584-95.  2010

显示摘要
20734311 20734311
Mitochondrial loss, dysfunction and altered dynamics in Huntington's disease.
Kim J, Moody JP, Edgerly CK, Bordiuk OL, Cormier K, Smith K, Beal MF, Ferrante RJ
Hum Mol Genet  2010

显示摘要
20660112 20660112
Long-term localized high-frequency electric stimulation within the myocardial infarct: effects on matrix metalloproteinases and regional remodeling.
Rupak Mukherjee,William T Rivers,Jean Marie Ruddy,Robert G Matthews,Christine N Koval,Rebecca A Plyler,Eileen I Chang,Risha K Patel,Christine B Kern,Robert E Stroud,Francis G Spinale
Circulation  122  2010

显示摘要 全文本文章
20566951 20566951
Temporal disparity in the induction of matrix metalloproteinases and tissue inhibitors of metalloproteinases after thoracic aortic aneurysm formation.
John R Barbour, Robert E Stroud, Abigail S Lowry, Leslie L Clark, Allyson M Leone, Jeffery A Jones, Francis G Spinale, John S Ikonomidis
The Journal of thoracic and cardiovascular surgery  132  788-95  2006

显示摘要
17000289 17000289
Glucose-induced changes in integrins and matrix-related functions in cultured human glomerular epithelial cells.
Kitsiou, Paraskevi V, et al.
Am. J. Physiol. Renal Physiol., 284: F671-9 (2003)  2003

显示摘要
12620921 12620921
Renal connective tissue growth factor correlates with glomerular basement membrane thickness and prospective albuminuria in a non-human primate model of diabetes: possible predictive marker for incipient diabetic nephropathy.
Sally E Thomson,Susan V McLennan,Paul D Kirwan,Scott J Heffernan,Annemarie Hennessy,Dennis K Yue,Stephen M Twigg
Journal of diabetes and its complications  22  2001

显示摘要
18413184 18413184
Overexpression of copper and zinc superoxide dismutase in transgenic mice prevents the induction and activation of matrix metalloproteinases after cold injury-induced brain trauma.
Morita-Fujimura, Y; Fujimura, M; Gasche, Y; Copin, JC; Chan, PH
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism  20  130-8  2000

显示摘要
10616801 10616801

数据表

标题
RABBIT ANTI-HUMAN TIMP-1