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05-184-AF488 Anti-Src Antibody, clone GD11, Alexa Fluor® 488 conjugate

Anti-Src Antibody, clone GD11, Alexa Fluor® 488 conjugate is an antibody against Src for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Oncoproteins Gene Symbol: V-SRC, SRC UniProt Number: P00526
05-184-AF488
100 µL  
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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
Av, R, M, H, MiICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue Number05-184-AF488
DescriptionAnti-Src Antibody, clone GD11, Alexa Fluor® 488 conjugate
Alternate Names
  • Tyrosine-protein kinase transforming protein Src
  • pp60v-src
  • p60-Src
  • v-Src
  • Proto-oncogene tyrosine-protein kinase Src
  • p60-Src
  • pp60c-src
  • Proto-oncogene c-Src
Background InformationTyrosine-protein kinase transforming protein Src (EC 2.7.10.2; UniProt P00526; also known as p60-Src, pp60v-src, v-Src) is encoded by the V-SRC gene (Gene ID 1491925) of Rous sarcoma virus strain Prague C (RSV-PrC).
Rous sarcoma virus (RSV) V-SRC gene is the first retroviral oncogene discovered. V-SRC encodes the v-Src tyrosine kinase that transforms infected host cells into uncontrolled mitosis, providing abundant cells for fresh infection. The non-oncogenic chicken c-Src (cellular Src) gene structurally related to v-Src was discovered later in 1979 by J. Michael Bishop and Harold E. Varmus. It is believed that RSV originally took up the chicken proto-oncogene SRC (Gene ID 396442; UniProt P00523) and incorporated into its genome in the mutated concogene form. c-Src contains six functional regions, Src homology 4 (SH4) domain, unique region, SH3 domain, SH2 domain, catalytic domain, and short regulatory tail. In its inactive state, Src is phosphorylated at Tyr527. The pTyr527 interacts with the SH2 domain which helps the SH3 domain interact with the linker domain and consequently keeps the inactive unit tightly bound. Dephosphorylation of pTyr527 destabilizes the inactive conformation, opening up the SH3, SH2 and the kinase domains, leading to Tyr416 autophosphorylation and c-Src activation.
References
Product Information
FormatAlexaFluor®488
PresentationPurified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Src Antibody, clone GD11, Alexa Fluor® 488 conjugate is an antibody against Src for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: An 1:100 dilution of this antibody from a representative lot detected Src in A431, HUVECs, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. MAB1618) is shown to be suitable also for Western blotting, immunocytochemistry, immunofluorescence, and immunoprecipitation applications.
Biological Information
ImmunogenRecombinant PrA-RSV p60src.
EpitopeAmino acids 82-169.
CloneGD11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone GD11 reacted with v-Src of Rous sarcoma virus (RSV) strains Prague subgroup A (PrA-RSV), Schmidt-Ruppin subgroup D (SRD-RSV), and Bratislava 77 subgroup C (B77 C-RSV). Clone GD11 detected enzymatically active c-Src from a variety of chicken, rodent, and human cells (Parsons, S.J., et al. (1984). J. Virol. 51(2):272-82).
IsotypeIgG1
Species Reactivity
  • Avian
  • Rat
  • Mouse
  • Human
  • Mink
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • V-SRC
  • SRC
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~60 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: An 1:100 dilution of this antibody detected Src in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
05-184-AF488 04055977330564