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MABE2037-100UL Anti-STAG1/SA1 Antibody, clone SUSI63B

Anti-STAG1/SA1, clone SUSI63B, Cat. No. MABE2037, is a rat monoclonal antibody that detects STAG1/SA1 and is tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

MABE2037-100UL
100 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABE2037-100UL
DescriptionAnti-STAG1/SA1 Antibody, clone SUSI63B
Alternate Names
  • Cohesin subunit SA-1 (UniProt: Q9D3E6
  • also known as SCC3 homolog 1
  • Stromal antigen 1
Background InformationCohesin subunit SA-1 (UniProt: Q9D3E6; also known as SCC3 homolog 1, Stromal antigen 1) is encoded by the Stag1 (also known as Sa1) gene (Gene ID: 20842) in murine species. Cohesin are known to mediate sister chromatid cohesion and 3D genome folding. They hold the sister chromatids together to ensure correct DNA repair by homologous recombination and proper chromosome segregation during cell division. They are essential for cell proliferation. The cohesin complex forms a large proteinaceous ring within which sister chromatids can be trapped and at anaphase stage. The complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. This complex may also play a role in spindle pole assembly during mitosis. Two variant cohesin complexes containing Structural Maintenance of Chromosomes (SMC) 1, SMC3, RAD21 and either STAG/SA1 or STAG2/SA2 are present in all cell types. Before prophase, STAG1/SA1 appears to be scattered along chromosome arms. However, during prophase, most of cohesin complexes dissociate from chromatin. This dissociation is probably due to its phosphorylation by PLK1. At anaphase, the RAD21 subunit is cleaved that leads to the dissociation of the complex from chromosomes, allowing their separation. (Ref.: De Koninck, M., et al. (2020). Cell Rep.32(6); 108014; Kojic, A., et al. (2018). Nat. Struct. Mol. Biol. 25(6); 496-504).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ200
Applications
ApplicationAnti-STAG1/SA1, clone SUSI63B, Cat. No. MABE2037, is a rat monoclonal antibody that detects STAG1/SA1 and is tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunohistochemistry
  • Flow Cytometry
Application NotesTested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected STAG1/SA1 in HeLa and RAW264.7 cell lysates.

Flow Cytometry Analysis: A representative lot detected STAG1/SA1 in Flow Cytometry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).

Immunofluorescence Analysis: A representative lot detected STAG1/SA1 in Immunofluorescence applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).

Western Blotting Analysis: A representative lot detected STAG1/SA1 in Western Blotting applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014; Kojic, A., et al. (2018). Nat Struct Mol Biol. 25(6): 496-504; Cuadrado, A., et al. (2019). Cell Rep. 27(12): 3500-3510.e4).

Immunohistochemistry Applications: A representative lot detected Stag1 in Immunohistochemistry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenMBP-tagged recombinant fragment corresponding to the first 225 amino acids from N-terminal region of mouse Cohesin subunit SA-1 (STAG1).
EpitopeN-terminal
CloneSUSI63B
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone SUSI63B is a rat monoclonal antibody that detects STAG1/SA1. It targets an epitope within the N-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Stag1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~145 kDa observed; 144.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected STAG1/SA1 in MCF-7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABE2037-100UL 04065268364512