Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABS1195 Anti-SSH1 Antibody, clone 12C6.1

Anti-SSH1 Antibody, clone 12C6.1 is an antibody against SSH1 for use in Western Blotting.

Anti-SSH1 Antibody, clone 12C6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: SSH1, KIAA1298, SSH1L UniProt Number: Q8WYL5
MABS1195
100 μg  
Purchase on Sigma-Aldrich

特卖

概述

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1195
DescriptionAnti-SSH1 Antibody, clone 12C6.1
Alternate Names
  • Protein phosphatase Slingshot homolog 1
  • hSSH-1L
  • Slingshot homolog 1
  • SSH-1L
  • SSH-like protein 1
Background InformationProtein phosphatase Slingshot homolog 1 (EC 3.1.3.16; EC 3.1.3.48; UniProt Q8WYL5; also known as hSSH-1L, Slingshot homolog 1, SSH-1L, SSH-like protein 1) is encoded by the SSH1 (also known as KIAA1298, SSH1L) gene (Gene ID 54434) in human. SSH1 is the founding member of the Slingshot family of protein phosphatases that function to destabilize the actin cytoskeleton by reversing cofilin Ser3 phosphorylation and thereby re-activating cofilin. Cofilin plays a central role in regulating actin filament turnover and actin cytoskeletal remodelling by severing and depolymerizing actin filaments. Cofilin activity is negatively regulated by LIM kinases-catalyzed Ser3 phosphorylation. The Slingshot family members include SSH-1L, SSH-2L, SSH-3L, and chronophin. The members differ in their subcellular distribution, F-actin-binding activity, and phosphatase activity, implying related but member-specific functions.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SSH1 Antibody, clone 12C6.1 is an antibody against SSH1 for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to an internal sequence of human SSH1.
EpitopeInternal.
Clone12C6.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with spliced isoform 1 (isoform L), isoform 2 (isoform S), isoform 4, and isoform 5, but not isoform 3 (isoform B) reported by UniProt (Q8WYL5).
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SSH1
  • KIAA1298
  • SSH1L
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~150 kDa observed. 115.5 kDa (isoform 1), 77.43 kDa (isoform 2), 80.68 kDa (isoform 4), and 78.98 kDa (isoform 5) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SSH1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABS1195 04055977330304