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MAB4400-I-25UL Anti-Renilla Luciferase Antibody, clone 5B11.2

Anti-Renilla Luciferase, clone 5B11.2, Cat. No. MAB4400-I, is a mouse monoclonal antibody that detects Renilla-luciferase and is tested for use in Affinity Binding Assay, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and WB.

MAB4400-I-25UL
25 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMAB4400-I-25UL
DescriptionAnti-Renilla Luciferase Antibody, clone 5B11.2
Alternate Names
  • Coelenterazine h 2-monooxygenase
  • EC:1.13.12.5
  • Renilla-luciferin 2-monooxygenase
Background InformationCoelenterazine h 2-monooxygenase (UniProt: P27652; also known as EC:1.13.12.5, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase) is a monomeric, bioluminescent protein derived from the sea pansy (Renilla reniformis). Due to its light emitting abilities, the luciferase from Renilla reniformis has been widely used in molecular biology as a reporter gene in cell culture experiments and small animal imaging. Renilla luciferase is shown to be present in membrane-bound intracellular structures within specialized light-emitting cells along with a closely interacting green fluorescent protein (RrGFP) and a calcium- activated luciferin-binding protein. Upon binding the substrate, it catalyzes an oxygenation reaction, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure that collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. The optimum pH for this reaction is 7.4. It is shown that under in vivo conditions the product undergoes the process of nonradiative energy transfer to an accessory protein, a GFP, which results in green bioluminescence (~ 509 nm). However, in vitro, in the absence of GFP, it emits blue light (~480 nm). (Ref.: Loening, AM., et al. (2007). J. Mol. Biol. 374(4); 1017-1028; Lorenz, WW., et al. (1991). Proc. Natl. Acad. Sci. USA 88(10); 4438-4442).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Renilla Luciferase, clone 5B11.2, Cat. No. MAB4400-I, is a mouse monoclonal antibody that detects Renilla-luciferase and is tested for use in Affinity Binding Assay, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and WB.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Enzyme Immunoassay (ELISA)
  • Immunoprecipitation
  • Affinity Binding Assay
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot detected Renilla Luciferase in Western Blotting applications (Fujikawa, Y., et. al. (2014). Rice (NY). 7(1):11).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Renilla Luciferase in Immunoprecipitation applications (Marcelli, F., et.al. (2014). PLoS One. 9(6):e100096).

Enzyme Immunoassay (ELISA) Analysis: A representative lot detected His-tagged recombinant Renilla Luciferase.

Immunofluorescence Analysis: A representative lot detected Renilla Luciferase in Immunofluorescence applications (Reyes-Resina, I., et.al. (2020). Cells. 9(1):152).

Immunocytochemistry Analysis: A representative lot detected Renilla Luciferase in Immunocytochemistry applications (Franco, R., et.al. (2020). Cells. 9(5):1075).

Affinity Binding Assay: A representative lot of this antibody bound Renilla Luciferase protein with a KD of 3.38 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged Luciferase from Renilla reniformis.
EpitopeUnknown
Clone5B11.2
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 5B11.2 is a mouse monoclonal antibody that detects Renilla-type luciferase (Renilla-luciferin 2-monooxygenase).
IsotypeIgG1
Species Reactivity
  • Invertebrates
Species Reactivity NotePredicted to react with Invertebrates based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
UniProt Number
Molecular Weight~ 49 kDa observed; 36.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged recombinant Renilla Luciferase.

Western Blotting Analysis: 1:1,000 dilution of this antibody detected N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged recombinant Renilla luciferase.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MAB4400-I-25UL 04065265324250