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MABN2428-25UL Anti-Reelin C-Term Antibody, clone 12C10

MABN2428-25UL
25 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABN2428-25UL
DescriptionAnti-Reelin C-Term Antibody, clone 12C10
Alternate Names
  • RELN-C-terminal
Background InformationReelin (UniProt: P78509; also known as RELN) is encoded by the RELN gene (Gene ID: 5649) in human. Reelin is a large extracellular matrix glycoprotein with serine protease activity. It is synthesized with a signal peptide (aa 1-25) that is subsequently cleaved off to produce the mature form that contains 8 EGF-like domains. Its Reelin domain is located in amino acids 26-190. Reelin plays a role in brain development and layering of neurons in the cerebral cortex and cerebellum. It is abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In the adult brain it is preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. It is also expressed in the retina, liver, thyroid gland, adrenal gland, fallopian tube, and breast tissue. Reelin is shown to regulate the positioning of neurons and participates in neurotransmission and memory formation. It also regulates microtubule function in neurons and neuronal migration and affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Reelin participates in canonical signaling via binding to VLDL receptor and apolipoprotein E receptor 2 and induces tyrosine phosphorylation of the adaptor protein disabled 1 (Dab1) by Src kinases. Phosphorylated Dab1 in-turn activates downstream signaling, including the PI3-kinase-dependent pathways. Mutations in RELN gene have been linked to familial temporal lobe epilepsy and Lissencephaly that is associated with ataxia, mental retardation, and seizures. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mikulska-Ruminska, K., et al. (2019). Sci. Rep. 9; 18974; Dlugosz, P. and Nimpf, J. (2018). J. Mol. Sci. 19(10); E3090).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-Reelin C-Term, clone 12C10, Cat. No. MABN2428, is a mouse monoclonal antibody that detects Reelin and is tested for use in Immunofluorescence, Immunohistochemistry, Inhibition Assay, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunohistochemistry
  • Inhibition
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Reelin C-Term in Westen Blotting applicatiions (Kohno, T., et. al (2015). J Neurosci. 35(11):4776-87).

Immunohistochemistry Analsyis: A representative lot detected Reelin C-Term in Immunohistochemistry applications (Kohno, T., et. al (2015). J Neurosci. 35(11):4776-87).

Immunohistochemistry Analsyis: A 1:500 diltion from a representative lot detected Reelin C-Term in mouse neocortex section (Courtesy of Takao Kohno, Ph.D., Nagoya City University, Japan).

Western Blotting Analysis: A 1:500 dilution from a represenative lot detected Reelin C-Term in Supernatants from HEK293T expressing mouse Reelin treated with Furin inhibitor-I (Courtesy of Takao Kohno, Ph.D., Nagoya City University, Japan).

Immunofluorescence Analysis: A representative lot detected Reelin C-Term in Immunofluorescence applications (Kohno, T., et. al (2015). J Neurosci. 35(11):4776-87).

Inhibition Analysis: A representative lot Inhibited the interaction between AP-RR78C-FL and the neuronal cell membrane. (Kohno, T., et. al (2015). J Neurosci. 35(11):4776-87).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Reelin.
EpitopeC-terminus
Clone12C10
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 12C10 is a mouse monoclonal antibody that detects Reelin. It targets an epitope within 17 amino acids from the C-terminal region.
IsotypeIgG2bκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RELN
Purification MethodProtein G purified
UniProt Number
Molecular Weight388.39 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.



Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABN2428-25UL 04061842378234

Documentation

Anti-Reelin C-Term Antibody, clone 12C10 MSDS

职位

物料安全数据表 (MSDS) 

Anti-Reelin C-Term Antibody, clone 12C10 分析证书

标题批号
Anti-Reelin C-Term, clone 12C10 - Q3484344 Q3484344

参考

参考概述公共医疗ID
Importance of Reelin C-terminal region in the development and maintenance of the postnatal cerebral cortex and its regulation by specific proteolysis.
Kohno T, Honda T, Kubo K, Nakano Y, Tsuchiya A, Murakami T, Banno H, Nakajima K, Hattori M.
J Neurosci  35(11)  4776-87  2015

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