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MABC606 Anti-RNF43 Antibody, clone 11C3.1

Detect RNF43 using this mouse monoclonal Anti-RNF43, clone 11C3.1 Antibody, Cat. No. MABC606, validated for use in Western Blotting.

Anti-RNF43 Antibody, clone 11C3.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: RNF43 UniProt Number: Q68DV7
MABC606
100 μg  
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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC606
DescriptionAnti-RNF43 Antibody, clone 11C3.1
Alternate Names
  • E3 ubiquitin-protein ligase RNF43
  • RING finger protein 43
Background InformationE3 ubiquitin-protein ligase RNF43 (UniProt Q68DV7; also known as RING finger protein 43) is encoded by the RNF43 gene (Gene ID 54894) in human. RNF43 and the homologous ZNRF3 are cell-surface transmembrane E3 ubiquitin ligases upregulated upon Wnt/ -catenin signaling and act as potent Wnt signaling pathway negative feedback regulators by mediating ubiquitination of Frizzled (FZD) and LRP6 for lysosomal degradation. RNF43/ZNRF3 activity is counteracted by stem cell growth factor R-spondin that induces membrane ZNRF3/RNF43 clearance by binding ZNRF3/RNF43 and LGR4/LGR5. Physical interaction with Dishevelled (DVL) is essential for ZNRF3/RNF43 Wnt inhibitory activity, and DVL knockdown significantly increases cell surface levels of FZD and LRP6. In addition, RNF43 suppresses canonical and noncanonical Wnt signaling via distinct mechanisms. While interaction between the extracellular protease-associated (PA) domain and the cysteine-rich domain (CRD) of FZD and RNF43 intracellular RING finger domain is necessary for canonical Wnt/ -catenin signaling inhibition, interaction between FZD PDZ domain and RNF43 C-terminal cytoplasmic region is essential for suppressing noncanonical Wnt signaling. R-spondin overexpression through gene translocation and loss-of-function RNF43/ZNRF3 gene mutations are found in human cancers as means to escape from Wnt signaling negative feedback regulation. Human RNF43 is produced with a signal peptide (a.a. 1-23) sequence, which is posttranslationally removed to yiled the mature enzyme with an N-terminal extracellular domain (a.a. 24-197), a transmembrane domain (a.a. 198-218), and a large cytoplasmic region (a.a. 219-783) that contains an atypical RING-type zinc finger (a.a. 272-313) and a pro-rich (a.a. 569-760) region.
References
Product Information
FormatPurified
PresentationPurified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect RNF43 using this mouse monoclonal Anti-RNF43, clone 11C3.1 Antibody, Cat. No. MABC606, validated for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant human RNF43 fragment from the cytoplasmic pro-rich region.
Clone11C3.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 11C3.1 epitope lies within the cytoplasmic pro-rich region present in all four spliced isoforms of human RNF43 reported by UniProt (Q68DV7).
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RNF43
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~95 kDa observed. Target band size appears larger than the calculated molecular weights of 83.20/85.72 kDa (isoform 1 mature/pro-form), 78.71/81.23 kDa (isoform 2 mature/pro-form), 72.11 kDa (isoform 3) 92.52/95.04 kDa (isoform 4 mature/pro-form) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected RNF43 in 10 µg of HCT116 human colon carcinoma cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABC606 04054839055072

Documentation

Anti-RNF43 Antibody, clone 11C3.1 MSDS

职位

物料安全数据表 (MSDS) 

Anti-RNF43 Antibody, clone 11C3.1 分析证书

标题批号
Anti-RNF43, clone 11C3.1 - 3393270 3393270
Anti-RNF43, clone 11C3.1 - 3666795 3666795
Anti-RNF43, clone 11C3.1 - 3854175 3854175
Anti-RNF43, clone 11C3.1 - 4069733 4069733
Anti-RNF43, clone 11C3.1 - 4126640 4126640
Anti-RNF43, clone 11C3.1 - 4194983 4194983
Anti-RNF43, clone 11C3.1 -Q2737194 Q2737194
Anti-RNF43, clone 11C3.1 Monoclonal Antibody 3009975
Anti-RNF43, clone 11C3.1 Monoclonal Antibody 2992363