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05-623-AF488 Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 488 Conjugate

This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: POLR2A, POLR2 UniProt Number: P24928
05-623-AF488
100 µL  
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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue Number05-623-AF488
DescriptionAnti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 488 Conjugate
Alternate Names
  • DNA-directed RNA polymerase II subunit RPB1
  • RNA polymerase II subunit B1
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase III largest subunit
  • RNA-directed RNA polymerase II subunit RPB1
  • RNA polymerase II
Background InformationDNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
References
Product Information
FormatAlexaFluor®488
PresentationPurified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in HeLa cells.
The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.
Biological Information
ImmunogenKLH-conjugated, synthetic peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
Epitopecarboxyl-terminal domain of RNA polymerase II
CloneCTD4H8
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityThis antibody recognizes Phospho- and non-phospho-RNA polymerase II.
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Yeast, Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • POLR2A
  • POLR2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~210-220 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
05-623-AF488 04055977298901

Documentation

Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 488 Conjugate MSDS

职位

物料安全数据表 (MSDS) 

Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 488 Conjugate 分析证书

标题批号
Anti-RNA polymerase II, clone CTD4H8, AlexaFluor® 488 Conjugate 2971792
Anti-RNA polymerase II, clone CTD4H8, AlexaFluor® 488 Conjugate 2899326
Anti-RNA polymerase II, clone CTD4H8, AlexaFluor® 488 Conjugate - 3318244 3318244
Anti-RNA polymerase II, clone CTD4H8, AlexaFluor® 488 Conjugate - 3709484 3709484
Anti-RNA polymerase II, clone CTD4H8, AlexaFluor® 488 Conjugate - 3898589 3898589
Anti-RNA polymerase II, clone CTD4H8, AlexaFluor® 488 Conjugate - 4187690 4187690
Anti-RNA polymerase II, clone CTD4H8, AlexaFluor® 488 Conjugate -Q2610524 Q2610524

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New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

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Post-Translational Modifications

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies