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MABF3184-100UG Anti-RANTES/CCL5 Antibody, clone 15B5.6

Anti-RANTES/CCL5, clone 15B5.6, Cat. No. MABF3184, is a mouse monoclonal antibody that detects RANTES/CCL5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

MABF3184-100UG
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABF3184-100UG
DescriptionAnti-RANTES/CCL5 Antibody, clone 15B5.6
Alternate Names
  • C-C motif chemokine 5
  • EoCP
  • Eosinophil chemotactic cytokine
  • SIS-delta
  • Small-inducible cytokine A5
  • T cell-specific protein P228
  • TCP228
  • T-cell-specific protein RANTES
Background InformationC-C motif chemokine 5 (UniProt: P13501; also known as EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, TCP228, T-cell-specific protein RANTES, CCL5) is encoded by the CCL5 (also known as D17S136E, SCYA5) gene (Gene ID: 6352) in human. CCL5 is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature protein (aa 24-91), which can further into RANTES (3-68) and RANTES (4-68). CCL5 serves as a chemoattractant for monocytes, memory T-helper cells, and eosinophils. It promotes immune cell recruitment to sites of inflammation. It also causes the release of histamine from basophils. It is involved in the activation of several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. The CCL5 activation of pancreatic cancer cells expressing CCR5 is reported to increase their invasive potential. CCL5 also functions as one of the natural ligands for the CCR5 and is reported to suppress in vitro replication of the R5 strains of HIV-1 that uses CCR5 as a coreceptor. The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a shown to be a potent inhibitor of HIV-1-infection. RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(3-68). (Ref.: Singh, SK., et al. (2018). Sci. Rep. 8; Article 1323; Lim, JK., et al. (2005). J. Leukoc Biol. 78(2); 442-452).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-RANTES/CCL5, clone 15B5.6, Cat. No. MABF3184, is a mouse monoclonal antibody that detects RANTES/CCL5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesTested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected RANTES/CCL5 in human spleen tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFull-length recombinant mature human RANTES/CCL5 (without the signal peptide).
EpitopeUnknown
Clone15B5.6
Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 15B5.6 is a mouse monoclonal antibody that detects RANTES/CCL5.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CCL5
Purification MethodProtein G purified
UniProt Number
Molecular Weight~8 kDa observed; 9.99 kDa calculated for the whole molecule. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant human RANTES/CCL5.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human RANTES/CCL5.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABF3184-100UG 04065267614007