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MABE343-AF647 Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate Antibody

This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair
MABE343-AF647
100 µL  
Purchase on Sigma-Aldrich

特卖

概述

特卖

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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCMAlexaFluor®647Monoclonal Antibody
Description
Catalogue NumberMABE343-AF647
DescriptionAnti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate Antibody
Background InformationPuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
Product Information
FormatAlexaFluor®647
PresentationPurified mouse conjugate in buffer containing PBS, 15 mg/mL BSA, and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.
Key Applications
  • Immunocytochemistry
Biological Information
ImmunogenBacteria expressing puromycin-incorporated neosynthesized proteins at multiple molecular weights.
Clone12D10
Concentration0.5 mg/mL
HostMouse
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with cells from all species when pre-incubated with puromycin.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
Molecular WeightRefer to Cat. No. MABE343 for observed molecular weight information.
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry Analysis untreated and puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected puromycin-incorporated neosynthesized proteins in puromycin treated HeLa cells.

Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
产品目录编号 GTIN
MABE343-AF647 04053252976308