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AB5374-50UL Anti-Potassium Channel Kir2.1 Antibody

Detect Potassium Channel Kir2.1 using this Anti-Potassium Channel Kir2.1 Antibody validated for use in IH & WB.

Anti-Potassium Channel Kir2.1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: KCNJ2, IRK1, HIRK1, Kir2.1, HHBIRK1, SQT3, HHIRK1, KIR2.1, LQT7 UniProt Number: P63252
AB5374-50UL
50 µL  
Purchase on Sigma-Aldrich

特卖

概述

Replacement Information

特卖

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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
RIHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5374-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Potassium Channel Kir2.1 Antibody
References
Product Information
FormatAffinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality LevelMQ100
Applications
ApplicationDetect Potassium Channel Kir2.1 using this Anti-Potassium Channel Kir2.1 Antibody validated for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern blot: 1:200 using ECL on rat brain and rat heart membranes. Note: Addition of 0.1% Tween to the standard milk block is recommended for this lot.

Immunohistochemistry on rat heart sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPurified peptide from human Kir2.1 (amino acids 392-410) (Accession number P48049).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Kir2.1 protein (IRK1, Kcnj2). The epitope is specific for Kir2.1 and is not present in any other known protein.

SPECIES REACTIVITIES: The immunogen sequence is identical in rabbit, bovine, porcine and guinea pig. It is highly conserved in rat and mouse (17/19 residues), chick (15/19 residues), and pigeon (14/18). Other species have not been tested.
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryPotassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Mutations in this gene have been associated with Andersen syndrome, which is characterized by periodic paralysis, cardiac arrhythmias, and dysmorphic features.
Gene Symbol
  • KCNJ2
  • IRK1
  • HIRK1
  • Kir2.1
  • HHBIRK1
  • SQT3
  • HHIRK1
  • KIR2.1
  • LQT7
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P63252 # Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium.
SIZE: 427 amino acids; 48288 Da
SUBUNIT: Homomultimeric and heteromultimeric association with Kir2.3, resulting in an enhanced G-protein-induced current. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Heart, brain, placenta, lung, skeletal muscle, and kidney. Diffusely distributed throughout the brain.
DISEASE: SwissProt: P63252 # Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7) [MIM:170390]; also called Andersen syndrome or Andersen cardiodysrhythmic periodic paralysis. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. LQT7 manifests itself as a clinical triad consisting of potassium-sensitive periodic paralysis, ventricular ectopy and dysmorphic features. & Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3) [MIM:609622]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SQT3 has a unique ECG phenotype characterized by asymmetrical T waves.
SIMILARITY: SwissProt: P63252 ## Belongs to the inward rectifier-type potassium channel family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
AB5374-50UL 04053252369186

Documentation

Anti-Potassium Channel Kir2.1 Antibody MSDS

职位

物料安全数据表 (MSDS) 

Anti-Potassium Channel Kir2.1 Antibody 分析证书

标题批号
RABBIT ANTI-Kir2.1 - 4182822 4182822
RABBIT ANTI-Kir2.1 -2594270 2594270
RABBIT ANTI-Kir2.1 -2611051 2611051
RABBIT ANTI-Kir2.1 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2969356
RABBIT ANTI-Kir2.1 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2956751
RABBIT ANTI-Kir2.1 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2975447
RABBIT ANTI-Kir2.1 AFFINITY PURIFIED POLYCLONAL ANTIBODY 3122259
RABBIT ANTI-Kir2.1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2172723 2172723
RABBIT ANTI-Kir2.1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2278527 2278527

数据表

标题
RABBIT ANTI-Kir2.1 AFFINITY PURIFIED POLYCLONAL ANTIBODY