Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABS1252 Anti-Polycystin-1 Antibody, clone E4 (5F4D2)

Anti-Polycystin-1 Antibody, clone E4 (5F4D2) is an antibody against Polycystin-1 for use in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting, Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: PKD1 UniProt Number: P98161
MABS1252
100 μg  
Purchase on Sigma-Aldrich

特卖

概述

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MIH(P), IP, WB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1252
DescriptionAnti-Polycystin-1 Antibody, clone E4 (5F4D2)
Alternate Names
  • Polycystin-1
  • Autosomal dominant polycystic kidney disease 1 protein
Background InformationPolycystin-1 (UniProt P98161; also known as Autosomal dominant polycystic kidney disease 1 protein) is encoded by the PKD1 gene (Gene ID 5310) in human. The primary cilium is a microtubule-based mechano- and chemo-sensory organelle that coordinates an array of cellular pathways during development and in tissue homeostasis, including Hedgehog (Hh), PDGFRα and Wnt signaling. Defective ciliary function causes a variety of ciliopathies, including autosomal dominant polycystic kidney disease (ADPKD). Polycystin-1 (PC1) and polycystin-2 (PC2) are large transmembrane proteins co-localized to primary cilium, where they play an important role in calcium-based signalling. PC1 is an atypical adhesion G-protein-coupled receptor (aGPCR) with 11-transmemrbane domains. PC1 is cis-autoproteolytically cleaved at a juxtamembrane GPCR cleavage site (GPS; a.a. 3012-3061) in a developmental stage-dependent manner, where PC1 is largely uncleaved in early embryonic kidneys but becomes extensively cleaved after birth. GPS cleavage results in a heterodimeric PC1 form, in which the N-terminal fragment (NTF) remains non-covalently associated with the transmembrane C-terminal fragment (CTF). Transgenic mice expressing non-cleavable PC1 develop cystic kidney disease during the postnatal period.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Polycystin-1 Antibody, clone E4 (5F4D2) is an antibody against Polycystin-1 for use in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting, Immunocytochemistry.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunohistochemistry Analysis: An 1:50 dilution of this antibody from a representative lot detected polycystin-1 immunoreactivity in rat and mouse kidney tissue sections.
Immunoprecipitation Analysis: A representative lot immunoprecipitated polycystin-1 from mouse lung tissue lysates (Courtesy of Dr. Feng Qian, University of Maryland School of Medicine, Baltimore, MD).
Western Blotting Analysis: A representative lot detected exogenously expressed polycystin-1 in lysates from HEK cells transfected with human or mouse Pkd1 cDNA, but not in lysates from mock-transfected HEK cells (Courtesy of Dr. Feng Qian, University of Maryland School of Medicine, Baltimore, MD).
Western Blotting Analysis: A representative lot detected the polycystin-1 (PC1) GPS domain cis-autocleaved N-terminal fragment (NTF) in lysates from HEK cells transfected with full-length Pkd1 cDNA, but not in lysates from mock-transfected HEK cells (Kim, H., et al. (2014). Nat. Commun. 5:5482).
Immunocytochemistry Analysis: A representative lot and an anti-Arl13b antibody co-stained the primary cilium of 4% formaldehyde-fixed, 0.1% Triton X-100-permeabilized murine embryonic fibrolasts (mMEF) and collecting duct (CD)-derived cells by dual fluorescence immunocytochemistry. No polycystin-1 immunoreactivity was detected in Pkd1-knockout mMEFs (Kim, H., et al. (2014). Nat. Commun. 5:5482).
Biological Information
ImmunogenFLAG-tagged recombinant human polycystin-1 C-type lectin domain.
EpitopeC-type lectin domain.
CloneE4 (5F4D2)
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone E4 (also known as clone 5F4D2) targets polycystin-1 extracellular C-type lectin domain and immunostained wild-type, but not Pkd1-knockout murine cells (Kim, H., et al. (2014). Nat. Commun. 5:5482).
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rat
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PKD1
Purification MethodProtein G Purified
UniProt Number
Molecular WeightCalculated molecular weights of full-length/GPS domain autocleaved N-terminal fragment: 460.3/325.0 kDa (isoform 1), 459.1/324.0 kDa (isoform 2), 460.2/325.0 kDa (isoform 3).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human kidney tissue.

Immunohistochemistry Analysis: An 1:250 dilution of this antibody lot detected polycystin-1 immunoreactivity in human kidney tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABS1252 04055977330298

Documentation

Anti-Polycystin-1 Antibody, clone E4 (5F4D2) MSDS

职位

物料安全数据表 (MSDS) 

Anti-Polycystin-1 Antibody, clone E4 (5F4D2) 分析证书

标题批号
Anti-Polycystin-1, clone E4 (5F4D2) - 3172411 3172411
Anti-Polycystin-1, clone E4 (5F4D2) - 3182194 3182194
Anti-Polycystin-1, clone E4 (5F4D2) - 3250987 3250987
Anti-Polycystin-1, clone E4 (5F4D2) - 3253297 3253297
Anti-Polycystin-1, clone E4 (5F4D2) - 3600581 3600581
Anti-Polycystin-1, clone E4 (5F4D2) - 3837016 3837016
Anti-Polycystin-1, clone E4 (5F4D2) - 4089936 4089936
Anti-Polycystin-1, clone E4 (5F4D2) - 4158995 4158995
Anti-Polycystin-1, clone E4 (5F4D2) -Q2658560 Q2658560
Anti-Polycystin-1, clone E4 (5F4D2) Monoclonal Antibody 3117580
Show more