Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN2727-100UG Anti-Pan Lhx1/5 Antibody, clone 4F2

Anti-Pan Lhx1/5, clone 4F2, Cat. No. MABN2727, is a mouse monoclonal antibody that detects Lhx1/5 and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

MABN2727-100UG
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABN2727-100UG
DescriptionAnti-Pan Lhx1/5 Antibody, clone 4F2
Alternate Names
  • LIM/homeobox protein Lhx5
Background InformationLIM/homeobox protein Lhx5 (UniProt: P61376; also known as LIM homeobox protein 5, Homeobox protein LIM-2) is encoded by the Lhx5 (also known as Lim-2, Lim2) gene (Gene ID: 124451) in rat. Lhx5 is a nuclear protein that belongs to a family of proteins that carry the LIM domain. Its LIM zinc-binding domains are localized in amino acids 3-61 and 62-125. It acts as a transcriptional regulator and is reported to play an essential role in the regulation of neuronal differentiation and migration during development of the central nervous system. Its expression is detected in developing Purkinje cells soon after they exited the cell cycle and migrate out of the cerebellar ventricular zone. Lhx5 is also required for the generation of the mamillary body (MM) and its derived tracts that are related to memory and spatial navigation that interconnect hippocampal, thalamic, and tegmental regions. It is predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Lhx5 is reported to regulate the local inhibition of Wnt proteins. Activation of Lhx5 is shown to expand forebrain structures. It can rescue forebrain deficiencies caused by excess Wnt activity. Its inhibition results in ectopic activation of Wnt signaling. It regulates the expression of two secreted Frizzled-related Wnt antagonists, Sfrp1a and Sfrp5 that can reduce the ectopic activation of Wnt signaling and rescue the forebrain deficiencies caused by inhibition of Lhx5 function. (Ref.: Miquelajáuregui, A., et al. (2015) Front. Neuroanat. 9; 136; Yang, M., et al. (2010). J. Comp. Neurol. 518(14); 2818-2840; Peng, G., and Westerfield, M. (2006). Development. 133(16); 3191-3200).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Pan Lhx1/5, clone 4F2, Cat. No. MABN2727, is a mouse monoclonal antibody that detects Lhx1/5 and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
Application NotesTested Applications

Immunocytochemistry Analysis: A representative lot detected Lhx1/5 in Immunocytochemistry applications (Iyer, N.R., et al. (2016). Exp Neurol. 277:305-316).

Immunohistochemistry Applications: A representative lot detected Pan Lhx1/5 in Immunohistochemistry applications (Lu, Y., et al. (2020). Dev Cell. 53(4):473_491.e9; Shirazi Fard, S., et al. (2014). Cell Cycle. 13(3):408-17; Hotani, T., et al. (2018). Mol Ther Oncolytics. 12:162-172; Yang, M., et al. (2010). J Comp Neurol. 518(14):2818-40).

Immunofluorescence Analysis: A representative lot detected Pan Lhx1/5 in Immunofluorescence applications (Yang, M., et al. (2010). J Comp Neurol. 518(14):2818-40; Samson, A., et al. (2018). Gut. 67(3):562-573).

Western Blotting Analysis: A representative lot detected Pan Lhx1/5 in Western Blotting applications (Hotani, T., et al. (2018). Mol Ther Oncolytics. 12:162-172).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenRecombinant rat Lim2/LhxV5 protein.
EpitopeUnknown
Clone4F2
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 4F2 is a mouse monoclonal antibody that detects.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Chicken
Species Reactivity NoteHuman, Mouse, Chicken, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Lhx1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~48 kDa observed; 44.78 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Lhx1/5 in Mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABN2727-100UG 04065270845405