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MABN2300-100UG Anti-PSMA/FOLH1 Antibody, clone 3F11

Anti-PSMA/FOLH1, clone 3F11, Cat. No. MABN2300, is a mouse monoclonal antibody that detects Prostate-specific membrane antigen (PSMA) and is tested in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

MABN2300-100UG
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABN2300-100UG
DescriptionAnti-PSMA/FOLH1 Antibody, clone 3F11
Alternate Names
  • Glutamate carboxypeptidase 2
  • EC:3.4.17.21
  • Cell growth-inhibiting gene 27 protein
  • Folate hydrolase 1
  • Folylpoly-gamma-glutamate carboxypeptidase
  • FGCP
  • Glutamate carboxypeptidase II
  • GCPII
  • Membrane glutamate carboxypeptidase
  • N-acetylated-alpha-linked acidic dipeptidase I
  • NAALADase I
  • Prostate-specific membrane antigen
  • PSM
  • Pteroylpoly-gamma-glutamate carboxypeptidase
Background InformationGlutamate carboxypeptidase 2 (UniProt: Q04609; also known as EC:3.4.17.21, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Glutamate carboxypeptidase II, GCPII, Membrane glutamate carboxypeptidase, mGCP, N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, Prostate-specific membrane antigen, PSM, PSMA, Pteroylpoly-gamma-glutamate carboxypeptidase) is encoded by the FOLH1 (also known as FOLH, NAALADI, PSM, PSMA, GIG27) gene (Gene ID: 2346) in human. PSMA is a single-pass type II membrane protein that has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase activities. It exhibits a preference for tri-alpha-glutamate peptides. Its expression pattern is restricted mainly to the healthy prostate secretory-acinar epithelium and the plasma membrane of epithelial prostate carcinoma. In the brain, it modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG) and release of glutamate. Clone 3F11 is shown to be highly specific for PSMA and does not exhibit any cross-reactivity with unrelated proteins. It binds to a linear epitope (aa 226-243) within the extracellular domain and is reported to be suitable for the detection of PSMA orthologs from some other species when denatured form of PSMA is used. (Ref.: Vahidfar, N., et al., (2019). J. Radioanal. Nucl. Chem. 322:237 248; Novakova, Z., et al. (2017). The Prostate 77(7); 749-764).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-PSMA/FOLH1, clone 3F11, Cat. No. MABN2300, is a mouse monoclonal antibody that detects Prostate-specific membrane antigen (PSMA) and is tested in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
  • ELISA
Application NotesTested Applications

Immunofluorescence Analysis: A representative lot detected PSMA in Immunofluorescence applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

ELISA Analysis: A representative lot detected PSMA in ELISA applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

Immunohistochemistry (Paraffin) Analysis: A 1:80,000 dilution from a representative lot detected PSMA in human prostate tissues (Courtesy of Barinka, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic and J. Hrudka, University Hospital Vinohrady, Prague, Czech Republic).

Immunohistochemistry Applications: A representative lot detected PSMA in Immunohistochemistry applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

Immunocytochemistry Analysis: A representative lot detected PSMA in Immunocytochemistry applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

Western Blotting Analysis: A representative lot detected PSMA in Western Blotting applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFull-length recombinant human Prostate-specific membrane antigen (PSMA).
EpitopeExtracellular domain
Clone3F11
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 3F11 is a mouse monoclonal antibody that detects Prostate-specific membrane antigen (PSMA). It targets an epitope within 18 amino acids from the extracellular domain.
IsotypeIgG1κ
Species Reactivity
  • Porcine
  • Mouse
  • Human
  • Rat
  • Canine
Species Reactivity NoteHuman, Canine, Mouse, Porcine, Rat.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • FOLH1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~120 kDa observed; 84.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in LNCap cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PSMA in LNCap cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABN2300-100UG 04065267404141