Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

07-293-I Anti-PICK1

07-293-I
100 µL  
Purchase on Sigma-Aldrich

特卖

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-293-I
DescriptionAnti-PICK1
Alternate Names
  • PRKCA-binding protein
  • Protein interacting with C kinase 1
  • Protein kinase C-alpha-binding protein
Background InformationPRKCA-binding protein (UniProt Q62083; also known as Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein) is encoded by the Pick1 (also known as Prkcabp) gene (Gene ID 18693) in murine species. PICK1 is a scaffolding protein expressed in many tissues, where it regulates trafficking of multiple membrane proteins. The PDZ (PSD-95/Dlg/ZO-1/DHR (Dlg homologous region) domain (a.a. 22-105) mediates PICK1 interaction with proteins containing PDZ-binding motifs, such as PKC and acid sensing ion channels (ASICs), while the lipid-binding AH (arfaptin homology)/BAR (Bin/amphiphysin/Rvs) domain (a.a. 144-357) mediates PICK1 homo-dimerization and membrane localization. Pick1 gene-disruption results in downregulated ASICs current and calcium transients during process of ischemia-reperfusion and protects against primary murine cortical neurons against injury caused by OGD-Rep (oxygen and glucose deprivation followed by reperfusion). PICK1 is also shown to act as a negatively regulator against ASIC1 phosphorylation/activation by PKA in rat pulmonary arterial smooth muscle cells, where PICK1 facilitates calcineurin-mediated ASIC1 dephosphorylation.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PICK1, Cat. No. 07-293-I, is a highly specific rabbit polyclonal antibody that targets PRKCA-binding protein and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PICK1 in human cerebellum, kidney, and pancreas tissue sections.
Biological Information
ImmunogenGST-tagged recombinant mouse PICK1 C-terminal fragment.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody targets the C-terminal region of mouse and rat PICK1, as well as human PICK1 spliced isoform 1, but not isoform 2, reported by UniProt (Q62083, Q9EP80, Q9NRD5).
Species Reactivity
  • Rat
  • Human
Species Reactivity NoteHuman, Rat. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Pick1
  • Prkcabp
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~55 kDa observed. 46.60 kDa (human/mouse) and 46.61 kDa (rat) calculated. The larger-than-calculated band size is consistent with that reported in the literature (Xia, J., et al. (1999). Neuron. 22(1):179 187).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PICK1 in 10 µg of PC12 rat pheochromocytoma cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
07-293-I 04054839057854