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MAB328-I-25UG Anti-Oligodendrocytes Antibody, clone CE-1

Anti-Oligodendrocytes, clone CE-1, Cat. No. MAB328-I, is a mouse monoclonal antibody that detects Oligodendrocytes and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

MAB328-I-25UG
25 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMAB328-I-25UG
DescriptionAnti-Oligodendrocytes Antibody, clone CE-1
Alternate Names
  • Myelin-oligodendrocyte glycoprotein
Background InformationOligodendrocytes are large glial cells found in the central nervous system. They are responsible for production of myelin sheath that insulates neuronal axons. The first step in the generation of oligodendrocytes is the emergence of oligodendrocyte precursors (OPCs) from embryonic radial glia. Subsequently, OPCs differentiate into immature oligodendrocytes before they begin to generate myelin. This process is regulated by both intrinsic and extrinsic signals in cells. Oligodendrocytes have only a brief period for myelination early during differentiation, and mature oligodendrocytes are incapable of myelinating. An essential signal for the onset of myelination is reported to be provided by the electrical activity of neurons. Although the ATP released from axons firing axon potentials does not directly act on OPCs or oligodendrocytes, it triggers the release of leukemia inhibitory factor (LIF) from astrocytes, which promotes myelination. Although the electrical activity of neurons is an essential promyelinating factor, other factors also come into play in this process. During development, all growing nerve fibers are shown to express the polysialylated-neural cell adhesion molecule (PSA-NCAM) that can prevent homophilic NCAM adhesion and cell-cell interactions. When this form of NCAM is downregulated, as it is in electrically active neurons, only then the process of myelination can proceed. (Ref.: Bradl, M., and Lassmann, H. (2010). Acta Neuropathol. 119(1); 37-53; Berto, S., et al. (2019). Proc. Natl. Acad. Sci. USA. 116(48); 24334-24342).
References
Product Information
FormatPurified
Control
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PresentationPurified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Oligodendrocytes, clone CE-1, Cat. No. MAB328-I, is a mouse monoclonal antibody that detects Oligodendrocytes and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Western Blotting
Application NotesTested applications

Western Blotting Analysis: A representative lot detected Oligodendrocytes in Western Blotting application (Goolsby, J., et al. (2003). Proc Natl Acad Sci USA. 100(25):14926-31).

Immunocytochemistry Analysis: A representative lot detected Oligodendrocytes in Immunocytochemistry application (Goolsby, J., et al. (2003). Proc Natl Acad Sci USA. 100(25):14926-31; Glavaski-Joksimovic, A., et al. (2009). Neuroscience. 162(2):472-81).

Immunofluorescence Analysis: A representative lot detected Oligodendrocytes in Immunofluorescence applications (Dubreuil, C., et al. (2003). J Cell Biol. 162(2):233-43; Glavaski-Joksimovic, A., et al. (2009). Neuroscience. 162(2):472-81; Wuthrich, C., et al. (2012). J Neuropathol Exp Neurol. 71(1):54-65).

Immunohistochemistry Applications: A representative lot detected Oligodendrocytes in Immunohistochemistry applications (Dubreuil, C., et al. (2003). J Cell Biol. 162(2):233-43; Glavaski-Joksimovic, A., et al. (2009). Neuroscience. 162(2):472-81; Wuthrich, C., et al. (2012). J Neuropathol Exp Neurol. 71(1):54-65).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenRat glial cell membrane proteins followed by rat CNS white matter.
EpitopeUnknown
CloneCE-1
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone CE-1 is a mouse monoclonal antibody that detects oligodendrocytes.
IsotypeIgM
Species Reactivity
  • Mouse
  • Rat
  • Human
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodLigatrap
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in Rat oligodendrocytes.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Rat Oligodendrocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MAB328-I-25UG 04065269048244