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MABS1254 Sigma-AldrichAnti-O-GlcNAc Antibody, clone CTD110.6

Anti-O-GlcNAc Antibody, clone CTD110.6 is an antibody against O-GlcNAc for use in Western Blotting, ELISA, Immunoprecipitation.

Anti-O-GlcNAc Antibody, clone CTD110.6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Signaling Research Sub-Category: General Post-translation Modification Gene Symbol: N/A UniProt Number: N/A

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概述

Replacement Information

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
A	WB, ELISA, IP	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABS1254
Description	Anti-O-GlcNAc Antibody, clone CTD110.6
Alternate Names	O-GlcNAc beta-O-GlcNAc O-Linked N-Acetylglucosamine beta-O-linked N-acetylglucosamine
Background Information	Posttranslational modification of proteins by β-linked N-acetylglucosamine (β-GlcNAc) via the hydroxyl moieties on serine or threonine residues is termed O-linked β-GlcNAc or simply O-GlcNAc. O-GlcNAc is one of the most abundant posttranslational modifications within the nucleocytoplasmic compartments of all animals and plants. Unlike other types of protein glycosylations, O-GlcNAc occurs exclusively within the nuclear and cytoplasmic compartments and is generally not further modified to form more elongated structures. In addition, O-GlcNAcylation is a highly dynamic and reversible process. The O-GlcNAc transferase (OGT) attaches O-GlcNAc to proteins at specific serine or threonine residues, while O-GlcNAcase catalyzes the removal/hydrolysis of O-GlcNAc from proteins. In fact, a dynamic interplay between O-GlcNAcylation and serine/threonine phosphorylation plays an important role in regulating cellular signaling. Tau and RNA polymerase II (Pol II) are two well known proteins that undergo modification by O-GlcNAcylation. In Alzheimer’s diseased human brains, tau becomes extensively phosphorylated and less O-GlcNAcylated. Similarly, O-GlcNAc is removed and replaced with O-phosphate on the Poly II CTD when the elongation phase of transcription is initiated.

References

Product Information
Format	Purified
HS Code	3002 15 90
Presentation	Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-O-GlcNAc Antibody, clone CTD110.6 is an antibody against O-GlcNAc for use in Western Blotting, ELISA, Immunoprecipitation.
Key Applications	Western Blotting ELISA Immunoprecipitation
Application Notes	Western Blotting Analysis: 1.0 µg/mL from a representative lot detected O-GlcNAcylated proteins in 7.5-15 µg of wild-type mouse embryonic fibroblast (MEF) lysate, but not O-GlcNAc transferase/OGT-deficient MEF lysate (Courtesy of Dr. Natasha Zachara and Gokben Yildirir, M.S.). ELISA Analysis: A representative lot detected RNA polymerase II C-terminal domain (CTD) peptide (YSPTSPS) with a single O-GlcNAcylated serine or threonine, but not the corresponding unmodified peptide (Comer, F.I., et al. (2001). Anal. Biochem. 293(2):169-177). Immunoprecipitation Analysis: A representative lot immunoprecipitated O-GlcNAcylated proteins from human pluripotent stem cells (hPSCs) (Maury, J.J., et al. (2013). Stem Cell Res. 11(2):926-937). Immunoprecipitation Analysis: A representative lot immunoprecipitated O-GlcNAcylated proteins from HeLa cell extracts (Comer, F.I., et al. (2001). Anal. Biochem. 293(2):169-177). Western Blotting Analysis: A representative lot detected similar level of cellular O-GlcNAcylation in undifferentiated, differentiating and terminally differentiated human pluripotent stem cells (hPSCs) (Maury, J.J., et al. (2013). Stem Cell Res. 11(2):926-937). Western Blotting Analysis: A representative lot detected BSA-conjugated RNA polymerase II C-terminal domain (CTD) peptide (YSPTSPS) with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc, nor the corresponding unmodified peptide. The presence of GlcNAc, but not GalNAc, abolished the detection of the target bands (Comer, F.I., et al. (2001). Anal. Biochem. 293(2):169-177). Western Blotting Analysis: A representative lot detected O-GlcNAcylated proteins in HeLa nuclear extract, as well as O-GlcNAcylated proteins purified from HeLa nuclear & cytosolic extract by wheat germ agglutinin (WGA) column. Antibody blocking by mmunogen peptide prior to immunoblotting abolished target bands detection (Comer, F.I., et al. (2001). Anal. Biochem. 293(2):169-177). Western Blotting Analysis: A representative lot detected an upregulation of O-GlcNAcylated proteins in Jurkat cells treated with the glucosaminidase inhibitor PUGNAc and the hexosamine pathway intermediate glucosamine (Comer, F.I., et al. (2001). Anal. Biochem. 293(2):169-177).

Biological Information
Immunogen	KLH-conjugated peptide with an O-GlcNAc-modified serine residue.
Epitope	beta-O-linked GlcNAc.
Clone	CTD110.6
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone CTD110.6 specifically detected serine and threonine residues with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc. The presence of GlcNAc, but not GalNAc, abolished the detection of the target modification. Clone CTD110.6 did not recognize peptides with unmodified serine and threonine residues (Comer, F.I., et al. (2001). Anal. Biochem. 293(2):169-177).
Isotype	IgMκ
Species Reactivity	All
Species Reactivity Note	All Species. Target modification is not species-specific.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	N/A
Gene Symbol	N/A
UniProt Number	N/A
Molecular Weight	Variable, depending on the size(s) of the O-GlcNAcylated protein(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 4.0 µg/mL of this antibody detected O-GlcNAcylated proteins in 10 µg of HeLa cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABS1254	04055977349610

Documentation

Anti-O-GlcNAc Antibody, clone CTD110.6 MSDS

职位
物料安全数据表 (MSDS)

Anti-O-GlcNAc Antibody, clone CTD110.6 分析证书

标题	批号
Anti-O-GlcNAc, clone CTD110.6 - 3237506	3237506
Anti-O-GlcNAc, clone CTD110.6 - 3280062	3280062
Anti-O-GlcNAc, clone CTD110.6 - 3485877	3485877
Anti-O-GlcNAc, clone CTD110.6 - 3590153	3590153
Anti-O-GlcNAc, clone CTD110.6 - 3722068	3722068
Anti-O-GlcNAc, clone CTD110.6 - 3811351	3811351
Anti-O-GlcNAc, clone CTD110.6 - 3907041	3907041
Anti-O-GlcNAc, clone CTD110.6 - 3932181	3932181
Anti-O-GlcNAc, clone CTD110.6 - 4146094	4146094
Anti-O-GlcNAc, clone CTD110.6 - 4209502	4209502

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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